CHARACTERIZATION OF PORIN AND OMPR MUTANTS OF A VIRULENT-STRAIN OF SALMONELLA-TYPHIMURIUM - OMPR MUTANTS ARE ATTENUATED INVIVO
CHARACTERIZATION OF PORIN AND OMPR MUTANTS OF A VIRULENT-STRAIN OF SALMONELLA-TYPHIMURIUM - OMPR MUTANTS ARE ATTENUATED INVIVO
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DOI：
10.1128/iai.57.7.2136-2140.1989
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发表时间：
1989-07-01
影响因子：
3.1
通讯作者：
DOUGAN, G
中科院分区：
文献类型：
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作者：
DORMAN, CJ;CHATFIELD, S;DOUGAN, G
OmpC、ompD和OmpF基因编码鼠伤寒沙门氏菌的三种主要蛋白。OmpR编码OMPC和OmpF表达所需的正调控因子。通过p22介导的转座子突变，将OMPC、ompD、OmpF和ompR转座子突变导入鼠伤寒沙门氏菌强毒株SL1344。在体外初步鉴定后，用这些菌株通过口服或静脉途径攻击BALB/c小鼠。携带OMPC或OmpF突变的菌株在口服攻击后的毒力与SL1344相同。携带ompD突变的菌株毒力略有下降。相比之下，ompR突变体在口服攻击后未能杀死BALB/c小鼠，静脉注射的50%致死剂量减少了约105。在口服或静脉注射攻击后，ompR突变体在小鼠组织中持续了几周。此外，口服免疫这些ompR突变株的小鼠能很好地抵抗强毒SL1344的攻击。
The ompC, ompD, and ompF genes encode the three major porins of Salmonella typhimurium. ompR encodes a positive regulator required for the expression of ompC and ompF. Transposon-generated mutations in ompC, ompD, ompF, and ompR were introduced into the S. typhimurium mouse virulent strain SL1344 by P22-mediated transduction. Following preliminary characterization in vitro, the strains were used to challenge BALB/c mice by using the oral or intravenous route. Strains harboring ompC or ompF mutations were as virulent as SL1344 after oral challenge. Strains harboring ompD mutations had a slight reduction in virulence. In contrast, ompR mutants failed to kill BALB/c mice after oral challenge and the intravenous 50% lethal dose was reduced by approximately 105. The ompR mutants persisted in murine tissues for several weeks following oral or intrevenous challenge. Furthermore, mice orally immuized with these ompR mutant strains were well protected against challenge with virulent SL1344.