Environmental DNA monitoring and management of invasive fish: comparison of eDNA and fyke netting
Environmental DNA monitoring and management of invasive fish: comparison of eDNA and fyke netting
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DOI：
10.3391/mbi.2017.8.1.09
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发表时间：
2017-03-01
影响因子：
1.5
通讯作者：
Gleeson, Dianne
中科院分区：
文献类型：
--
作者：
Hinlo, Rheyda;Furlan, Elise;Gleeson, Dianne
环境DNA （eDNA）方法在常规监测中增强入侵物种检测的潜力引起了管理机构的兴趣。在两个常规监测季节，我们采用eDNA方法结合传统检测技术，检测了澳大利亚三种具有不同空间分布（底栖、远洋和底栖）的入侵鱼类的存在：鲤鱼（Cyprinus carpio）、红鳍鲈（Perca fluviatilis）和东方气象泥鳅（Misgurnus anguillicaudatus）。我们的目标是比较使用eDNA和传统检测（fyke网）对目标物种的季节性检测，确定单位努力捕鱼量（CPUE）与DNA拷贝数之间的关系，并确定eDNA检测的最佳水域位置（地表与地下）。结果表明，在秋季和春季调查中，eDNA对东方风泥鳅和红鳍鲈的检出率均高于fke网。在两个季节的所有地点，使用fke网和eDNA都检测到鲤鱼，只有一个地点在秋季调查期间使用fke网捕获了普通鲤鱼，但没有检测到鲤鱼eDNA。季节对鲤鱼DNA浓度有显著影响
The potential of environmental DNA (eDNA) methods to enhance the detection of invasive species during routine monitoring is of interest to management agencies. Here we applied the eDNA methodology concurrent with conventional detection techniques during two routine monitoring seasons to detect the presence of three invasive fish in Australia with contrasting spatial distributions (benthopelagic, pelagic and benthic): common carp (Cyprinus carpio), redfin perch (Perca fluviatilis) and Oriental weatherloach (Misgurnus anguillicaudatus). Our objectives were to compare the seasonal detection of the target species using eDNA and conventional detection (fyke nets), determine the relationship between catch per unit effort (CPUE) and DNA copy number and ascertain the best water location (surface vs. subsurface) for eDNA detection. Our results show that eDNA had a higher detection rate than fyke nets for Oriental weatherloach and redfin perch during both the autumn and spring surveys. Common carp was detected at all sites for both seasons using fyke nets and eDNA with the exception of one site during the autumn survey where common carp was captured using fyke nets but no carp eDNA was detected. Season had a significant effect on DNA concentration for common carp (P