CsPM5.2, a phosphate transporter protein-like gene, promotes powdery mildew resistance in cucumber
CsPM5.2, a phosphate transporter protein-like gene, promotes powdery mildew resistance in cucumber
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DOI：
10.1111/tpj.16576
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发表时间：
2023-12-04
期刊：
影响因子：
7.2
通讯作者：
Wang，Gang
中科院分区：
文献类型：
--
作者：
Sun，Jingxian;Nie，Jingtao;Wang，Gang
白粉病是危害黄瓜最严重的真菌病害之一。黄瓜抗PM的机制复杂且不完整，受多个基因控制。在本研究中，我们通过QTL定位，检测到与PM5.2相关的主要效应QTL （Quantitative Trait Locus）。通过精细定位，克隆了抗性优势基因CsPM5.2，并通过转基因互补和自然变异鉴定证实了其功能。在品种9930中，由于编码区单核苷酸多态性导致cspm5.2突变体功能失调，并使其对磷酸易感性。cspm5.2编码磷酸转运蛋白PHO1；H3。cspm5.2的表达普遍存在，并受PM病原菌诱导。在黄瓜中，cspm5.2和cspm5.1 （Csmlo1）都是抗PM的必需基因。转录组分析表明，水杨酸（SA）途径可能在spm5.2介导的PM抗性中发挥重要作用。我们的研究结果有助于解析黄瓜抗PM的机制，并为培育抗PM的黄瓜品种提供策略。
Powdery mildew (PM) is one of the most serious fungal diseases affecting cucumbers (Cucumis sativusL.). The mechanism of PM resistance in cucumber is intricate and remains fragmentary as it is controlled by several genes. In this study, we detected the major‐effect Quantitative Trait Locus (QTL),PM5.2, involved in PM resistance by QTL mapping. Through fine mapping, the dominant PM resistance gene,CsPM5.2, was cloned and its function was confirmed by transgenic complementation and natural variation identification. In cultivar 9930, a dysfunctionalCsPM5.2mutant resulted from a single nucleotide polymorphism in the coding region and endowed susceptibility to PM.CsPM5.2encodes a phosphate transporter‐like protein PHO1; H3. The expression ofCsPM5.2is ubiquitous and induced by the PM pathogen. In cucumber, bothCsPM5.2andCspm5.1(Csmlo1) are required for PM resistance. Transcriptome analysis suggested that the salicylic acid (SA) pathway may play an important role inCsPM5.2‐mediated PM resistance. Our findings help parse the mechanisms of PM resistance and provide strategies for breeding PM‐resistant cucumber cultivars.