Changes of nitrogen-removal performance and that of the bacterial community in a mixed culture comprising freshwater and marine anammox bacteria under averaged environmental condition.
Changes of nitrogen-removal performance and that of the bacterial community in a mixed culture comprising freshwater and marine anammox bacteria under averaged environmental condition.
复制标题
淡水和海洋厌氧氨氧化菌混合培养物在平均环境条件下脱氮性能和细菌群落的变化。
DOI：
10.1016/j.chemosphere.2018.11.047
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发表时间：
2019
期刊：
影响因子：
8.8
通讯作者：
Hiroaki Ito
中科院分区：
文献类型：
--
作者：
Y. Kawagoshi;Yukiyasu Yamashita;Luong Van Duc;T. Hama;Hiroaki Ito
利用厌氧氨氧化细菌的脱氮工艺有望在保持经济的同时实现高效率的脱氮，并对其实际应用进行了研究。然而，厌氧氨氧化细菌仍然具有不利于实际使用的特性，包括对环境条件变化的敏感性。为了探索混合厌氧氨氧化细菌对环境条件的适应性，采用连续搅拌釜式反应器(CSTR)研究了淡水厌氧氨氧化细菌(FAB)和海洋厌氧氨氧化细菌(MAB)混合培养过程中脱氮性能和细菌群落的变化。接种混合厌氧氨氧化细菌的CSTR在淡水和海洋条件下运行180d，温度27.5 °C，使用含15 g/L氯化钠的合成介质。114d后脱氮效果趋于稳定，在0.07~0.42kgN/m~3/d的氮负荷范围内，90%以上的氮被去除。在0.42 kg N/m~3/d的条件下运行一个月后，采集生物量样品，利用16S rRNA的部分序列进行克隆文库分析。用Anammox细菌特异性引物获得的Anammox细菌克隆中，MAb占97%，Fab仅占3%。这些结果表明，包括厌氨氧化细菌在内的细菌群落因环境条件而发生了明显的变化，本研究的平均条件适合于海洋细菌，而不是淡水细菌。
Nitrogen-removal processes using anammox bacteria are expected to achieve high-rate removal while remaining economical, and their practical applications have been investigated. However, anammox bacteria still have unfavorable characteristics for practical use, including susceptibility to a change in environmental conditions. In this study, with an aim of exploring the adaptability of mixed anammox bacteria to environmental conditions, the shift of nitrogen-removal performance and bacterial community in a mixed culture comprising freshwater anammox bacteria (FAB) and marine anammox bacteria (MAB) were investigated by a continuously stirred tank reactor (CSTR). The CSTR inoculated with the mixed anammox bacteria was operated for 180 days under an averaged condition between freshwater and marine conditions with a temperature of 27.5 °C and a synthetic medium with 15 g/L NaCl was used. Nitrogen-removal performance became stable after 114 days and more than 90% of nitrogen that was loaded into the reactor was removed in the range of nitrogen loading rate 0.07–0.42 kg N/m3/d. After operating at 0.42 kg N/m3/d for one month, a biomass sample was taken and its bacterial community was analyzed by clone-library analysis using a partial sequence of 16S rRNA. Among the clones of anammox bacteria that were made by an anammox-bacteria-specific primer, 97% of them were MAB and only 3% were FAB. These results indicate that the bacterial community including anammox bacteria was evidently changed due to environmental conditions and that the averaged condition in this study was suitable for marine bacteria rather than freshwater bacteria.