Domain function and relevant enzyme activity of cycloinulooligosaccharide fructanotransferase from Paenibacillus polymyxa
Domain function and relevant enzyme activity of cycloinulooligosaccharide fructanotransferase from Paenibacillus polymyxa
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多粘类芽孢杆菌环寡糖果糖转移酶的结构域功能及相关酶活性
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
H. Kwon
中科院分区：
文献类型：
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作者：
M. Ko;K. You;Kwang;Byung;H. Kwon
环聚糖低聚果糖转移酶（CFTase）将菊糖转化为-连接的d -聚呋喃糖的环聚糖（cyclofructan， CF），并水解环聚糖。序列分析表明，CFTase在n端和c端被分成5个不同的区域，包含3个重复序列（R1、R3和R4）。通过比较野生型CFTase （CFT148）和缺失突变蛋白（CFT108: R1和R3缺失；CFT130: R4缺失；CFT88: R1、R3和R4缺失）来研究CFTase的各结构域功能。CFT108突变体与CFT148一样具有CFTase和CF水解活性。CFT130和CFT88突变体中CFTase活性和CF水解活性消失。这些结果表明，P. polymyxa CFTase的c端R4区域是环化和水解活性所必需的。
Cycloinulooligosaccharide fructanotransferase (CFTase) converts inulin into cycloinulooligosaccharides (cyclofructan, CF) of -linked D-fructofuranose as well as hydrolysis of cyclofructan. Sequences analysis indicated that CFTase was divided into five distinct regions containing three repeated sequences (R1, R3, and R4) at the N-terminus and C-terminus. Each domain function was investigated by comparison of wild type CFTase enzyme (CFT148) and deletion mutant proteins (CFT108: R1 and R3 deletion; CFT130: R4 deletion; and CFT88: R1, R3, and R4 deletion) of CFTase. The CFT108 mutant had both CFTase and CF hydrolyzing activity as CFT148 did. CFTase activities and CF hydrolysing activities were disappeared in CFT130 and CFT88 mutants. These results indicated that the C-terminal R4 region of P. polymyxa CFTase is necessary for cyclization and hydrolyzing activity.