ALDOSTERONE INHIBITS NITRIC-OXIDE SYNTHESIS IN RAT VASCULAR SMOOTH-MUSCLE CELLS INDUCED BY INTERLEUKIN-1-BETA
ALDOSTERONE INHIBITS NITRIC-OXIDE SYNTHESIS IN RAT VASCULAR SMOOTH-MUSCLE CELLS INDUCED BY INTERLEUKIN-1-BETA
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DOI：
10.1016/0922-4106(95)90018-7
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发表时间：
1995-07-18
期刊：
影响因子：
--
通讯作者：
SHIMADA, K
中科院分区：
文献类型：
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作者：
IKEDA, U;KANBE, T;SHIMADA, K
我们研究了醛固酮对血管平滑肌细胞一氧化氮(NO)合成的影响。我们测定了培养的大鼠血管平滑肌细胞中NO的稳定代谢产物亚硝酸盐的产生，以及诱导型NO合成酶的mRNA和蛋白的表达。加入IL-1β(10 ng/ml)培养24小时，可显著增加亚硝酸盐的生成。醛固酮以剂量(10(-9)与10(-6)M)依赖的方式显著抑制IL-1β诱导的血管平滑肌细胞亚硝酸盐的产生。与IL-1β共同孵育12h可诱导血管平滑肌细胞一氧化氮合酶mRNA表达，而醛固酮对其表达有抑制作用。醛固酮还可减少白介素1β诱导的诱导型一氧化氮合酶蛋白积聚。这些结果表明，在白介素1β刺激的条件下，醛固酮抑制血管平滑肌细胞中NO的合成。
We investigated the effects of aldosterone on nitric oxide (NO) synthesis in vascular smooth muscle cells. We measured the production of nitrite, a stable metabolite of NO, and the expression of inducible NO synthase mRNA and protein in cultured rat vascular smooth muscle cells. incubation of the cultures with interleukin-1 beta (10 ng/ml) for 24 h caused a significant increase in nitrite generation. The interleukin-1 beta-induced nitrite production by vascular smooth muscle cells was significantly inhibited by aldosterone in a dose (10(-9) similar to 10(-6) M)-dependent manner. Incubation with interleukin-1 beta for 12 similar to 24 h caused inducible NO synthase mRNA expression in vascular smooth muscle cells, whereas aldosterone had a suppressive effect on its expression. Aldosterone also decreased interleukin-1 beta-induced inducible NO synthase protein accumulation. These results indicate that aldosterone inhibits NO synthesis under interleukin-1 beta-stimulated conditions in vascular smooth muscle cells.