Detection of human herpesvirus 6 DNAs in samples from several body sites of patients with exanthem subitum and their mothers by polymerase chain reaction assay
Detection of human herpesvirus 6 DNAs in samples from several body sites of patients with exanthem subitum and their mothers by polymerase chain reaction assay
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聚合酶链式反应检测急疹患者及其母亲多个身体部位样本中的人类疱疹病毒 6 DNA
DOI：
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发表时间：
1995
影响因子：
12.7
通讯作者：
Y. Asano
中科院分区：
文献类型：
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作者：
S. Suga;T. Yazaki;Y. Kajita;T. Ozaki;Y. Asano
应用聚合酶链反应扩增技术检测了3例宫颈癌患者外周血单核细胞（MNCs）、血浆、唾液、粪便和尿液以及患者母亲外周血单核细胞、血浆和唾液中的人疱疹病毒6 (HHV‐6)dna。在疾病期间和之后的MNCs中检测到HHV - 6 dna，仅在急性期的血浆中发现。在疾病恢复后的唾液中也检测到病毒dna，在发病后的粪便中持续或间歇性地发现病毒dna，但在尿液样本中未发现病毒dna。相比之下，三个母亲中的一个在唾液中持续分泌HHV - 6 dna。没有母亲在外周血MNCs和血浆中检测到病毒dna，也没有母亲在可能从子女那里接触到HHV - 6病毒后抗体滴度显著增加。这些发现表明，在麻疹患者的急性期，HHV - 6的系统性复制和疾病恢复后病毒在几个器官的持续感染。©1995 Wiley‐Liss, Inc。
Polymerase chain reaction amplification was used to detect human herpesvirus 6 (HHV‐6) DNAs in peripheral blood mononuclear cells (MNCs), plasma, saliva, stool, and urine from three patients with exanthem subitum and in peripheral blood MNCs, plasma, and saliva from their mothers. HHV‐6 DNAs were detected in MNCs during and after the disease and were found in plasma only in the acute phase. The virus DNAs were also detected in saliva after recovery from the illness and were found persistently or intermittently in stool but not in urine samples after the onset of the disease. In contrast, one of the three mothers excreted HHV‐6 DNAs persistently in saliva. None of the mothers had the virus DNAs in peripheral blood MNCs and plasma nor a significant increase in antibody titers to HHV‐6 after possible exposure from their children. These findings suggest systemic replication of HHV‐6 during the acute phase in patients with exanthem subitum and persistent infection of the virus in several organs after recovery from the disease. © 1995 Wiley‐Liss, Inc.