In vitro effects of bisphenol A on the quality parameters, oxidative stress, DNA integrity and adenosine triphosphate content in sterlet (Acipenser ruthenus) spermatozoa
In vitro effects of bisphenol A on the quality parameters, oxidative stress, DNA integrity and adenosine triphosphate content in sterlet (Acipenser ruthenus) spermatozoa
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DOI：
10.1016/j.cbpc.2013.05.002
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发表时间：
2013-08-01
影响因子：
3.9
通讯作者：
Linhartova, Pavia
中科院分区：
文献类型：
--
作者：
Hulak, Martin;Gazo, Ievgeniia;Linhartova, Pavia
在内分泌干扰物中，由于人类广泛接触，异雌激素双酚A（BPA）值得特别关注。除了激素效应外，BPA还被怀疑对各种物种的生殖能力产生不利影响。本文研究了BPA对体外培养的鲟鱼精子的质量参数、氧化应激、DNA完整性和细胞内ATP含量的影响。将鱼精子暴露于可能存在于自然界中的BPA浓度（0.5、1.75、25、5和10 μ g/L）中2小时。结果表明，EPA浓度为2.5-10 μ g/L时，BPA显著降低精子的活动力和运动速度。运动精子百分率与ATP含量呈显著正相关（r = 0.713，P < 0.05）。在浓度为1.75-10微克/升时观察到氧化应激，这反映在蛋白质和脂质氧化水平以及超氧化物歧化酶活性明显较高。精子细胞内ATP含量随BPA浓度的增加而降低。在1.75 pg/L及以上浓度下，记录到DNA片段化显著增加，表示为尾DNA百分比（22% +/- 0.46）和Olive尾矩（0.37 +/- 0.09任意单位）。目前的研究证实，在自然界中可能遇到的BPA浓度能够诱导氧化应激，导致精子质量受损，DNA片段化和细胞内ATP含量。(C)2013年由Elsevier Inc.出版
Among endocrine disruptors, the xenoestrogen bisphenol A (BPA) deserves particular attention due to widespread human exposure. Besides hormonal effects, BPA has been suspected to be responsible for adverse effect on reproductive ability of various species. In the present study the effect of BPA on the quality parameters, oxidative stress, the DNA integrity and intracellular ATP content of sterlet (Acipenser ruthenus) spermatozoa were investigated in vitro. Fish spermatozoa were exposed to concentrations of BPA possibly occurring in nature (0.5, 1.75, 25, 5 and 10 mu g/L) for 2 h. Results revealed that BPA significantly decreased spermatozoa motility and velocity of spermatozoa at concentration of EPA 2.5-10 mu g/L. Significant positive correlation (r = 0.713, P < 0.05) was found between percent motile spermatozoa and ATP content. Oxidative stress was observed at concentrations 1.75-10 mu g/L, as reflected by significantly higher levels of protein and lipid oxidation and superoxide dismutase activity. Intracellular ATP content of spermatozoa decreased with increasing concentrations of BPA. A dramatic increase in DNA fragmentation expressed as percent tail DNA (22% +/- 0.46) and Olive tail moment (0.37 +/- 0.09 arbitrary units) was recorded at concentrations of 1.75 pg/L and above. The present study confirms that concentrations of BPA that can be encountered in nature are capable to induce oxidative stress, leading to impaired sperm quality, DNA fragmentation and intracellular ATP content. (C) 2013 Published by Elsevier Inc.