Effects of three-layered nanodisk size on cell detection sensitivity of plasmon resonance biosensors.
Effects of three-layered nanodisk size on cell detection sensitivity of plasmon resonance biosensors.
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DOI：
10.1016/j.bios.2015.07.022
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发表时间：
2015-12
影响因子：
12.6
通讯作者：
Xinghai Zhao;M. M. Wong-M.;S. Chiu;S. Pang
中科院分区：
文献类型：
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作者：
Xinghai Zhao;M. M. Wong-M.;S. Chiu;S. Pang
采用Au纳米盘（ND）和上下Au层之间的SiO2间隔层构建了双共振等离子体生物传感器，并将其用于检测MCF-7乳腺癌细胞。观察到Au纳米粒子的局域表面等离子体共振和纳米粒子与其下的Au膜差距耦合共振之间的杂化模式。这些多金属层ND表现出比普通的单金属层ND更高的灵敏度。消光光谱显示出双共振带，可以通过改变ND的大小来定制。多层ND的三种尺寸范围为60至200 nm直径（dia.）并分析它们对周围介质的折射灵敏度以用于细胞检测。直径为120的纳米盘显示出最高的折射灵敏度高达230 nm/折射率单位。这些传感器可用于检测从1.0× 103 cells/ml的低细胞浓度到1.7× 107 cells/ml的高细胞浓度的宽范围的MCF-7细胞。
The double resonance plasmonic biosensors based on Au nanodisks (NDs) with a thin SiO2spacer between the top and bottom Au layers were employed to detect MCF-7 breast cancer cells. The hybridized modes between the localized surface plasmon resonance of Au NDs and the gap coupling resonance of NDs with the Au film underneath have been observed. These multiple metallic layer NDs exhibit higher sensitivity than the common single metallic layer NDs. The extinction spectra showed double resonance bands that could be tailored by varying the ND size. Three sizes of multiple layer NDs ranging from 60 to 200 nm diameter (dia.) were generated and their refractive sensitivity to the surrounding media were analyzed for cell detection. Nanodisks with 120 dia. showed the highest refractive sensitivity up to 230 nm/refractive index unit. These sensors could be used to detect a broad range of MCF-7 cells from a low cell concentration down of 1.0×103cells/ml up to a high cell concentration of 1.7×107cells/ml.