The discovery of new intron-containing human tRNA genes using the polymerase chain reaction.
The discovery of new intron-containing human tRNA genes using the polymerase chain reaction.
复制标题
利用聚合酶链式反应发现新的含有内含子的人类 tRNA 基因。
DOI：
10.1016/s0021-9258(19)38321-8
复制
发表时间：
1990
影响因子：
4.8
通讯作者：
B. Vold
中科院分区：
文献类型：
--
作者：
C. Green;I. Sohel;B. Vold
转RNA基因中的内含子在脊椎动物中是罕见的。到目前为止，唯一含有内含子的人类tRNA基因被认为是编码tRNA（Tyr）的基因。所有这些内含子都插入到这些基因中反密码子位置的3'端。我们设计了聚合酶链反应引物，可以扩增所有的tRNA（Tyr）基因的克隆和测序，通过使用保守的部分编码的tRNA的结构部分的基因。我们的初步结果显示了五个tRNA（Tyr）基因，每个基因都包含一个不同的内含子。我们使用相同的技术扩增、克隆和测序人类tRNA（Leu）CAA基因。这导致发现该人类tRNA基因家族也具有插入反密码子3'的内含子。这种聚合酶链反应技术可用于检测含内含子的tRNA基因的新家族以及鉴定单个基因内含子中的序列变异。
Introns in transfer RNA genes are rare in vertebrates. Until now, the only intron-containing human tRNA genes were believed to be those coding for tRNA(Tyr). All of these introns are inserted 3' to the anticodon position in these genes. We have designed polymerase chain reaction primers that can amplify all of the tRNA(Tyr) genes for cloning and sequencing by using the conserved portions of the gene coding for the structural part of the tRNA. Our preliminary results have revealed five tRNA(Tyr) genes, each of which contains a different intron. We used the same technique to amplify, clone, and sequence the human genes for tRNA(Leu)CAA. This has resulted in the discovery that this human tRNA gene family also has introns inserted 3' to the anticodon. This polymerase chain reaction technique is useful in detecting new families of intron-containing tRNA genes as well as identifying sequence variations in the introns of individual genes.