Memory B cells contribute to rapid Bcl6 expression by memory follicular helper T cells
Memory B cells contribute to rapid Bcl6 expression by memory follicular helper T cells
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DOI：
10.1073/pnas.1404671111
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发表时间：
2014-08-12
影响因子：
11.1
通讯作者：
Kurosaki, Tomohiro
中科院分区：
文献类型：
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作者：
Ise, Wataru;Inoue, Takeshi;Kurosaki, Tomohiro
在原发性体液应答中，B细胞淋巴瘤6（Bcl 6）是滤泡辅助性T（T-FH）细胞分化的主要调节者;然而，其激活机制和在记忆应答中的作用仍不清楚。在这里，我们证明了CXCR 5（+）T-FH记忆细胞的存活以及随后的回忆抗体应答需要Bcl 6表达。此外，我们表明，在再挑战与可溶性抗原Bcl 6在记忆T-FH细胞迅速诱导的树突状细胞的独立方式和肽：II类复合物（pMHC）的同源记忆B细胞显着有助于这种诱导。鉴于先前的证据表明，抗原特异性B细胞驻留在卵泡获得抗原的几分钟内注射，我们的研究结果表明，记忆B细胞的同源T-FH记忆细胞的抗原，从而有助于快速Bcl 6的再表达和分化的T-FH记忆细胞在体液记忆反应。
In primary humoral responses, B-cell lymphoma 6 (Bcl6) is a master regulator of follicular helper T (T-FH) cell differentiation; however, its activation mechanisms and role in memory responses remain unclear. Here we demonstrate that survival of CXCR5(+) T-FH memory cells, and thus subsequent recall antibody response, require Bcl6 expression. Furthermore, we show that, upon rechallenge with soluble antigen Bcl6 in memory T-FH cells is rapidly induced in a dendritic cell-independent manner and that peptide: class II complexes (pMHC) on cognate memory B cells significantly contribute to this induction. Given the previous evidence that antigen-specific B cells residing in the follicles acquire antigens within minutes of injection, our results suggest that memory B cells present antigens to the cognate T-FH memory cells, thereby contributing to rapid Bcl6 reexpression and differentiation of the T-FH memory cells during humoral memory responses.