Preparation of intercalating dye thiazole orange and derivatives
Preparation of intercalating dye thiazole orange and derivatives
复制标题
DOI：
10.1016/0143-7208(95)00056-9
复制
发表时间：
1995-01-01
影响因子：
4.5
通讯作者：
Sabnis, RW
中科院分区：
文献类型：
--
作者：
Deligeorgiev, TG;Gadjev, NI;Sabnis, RW
描述了一种通过 6-取代-2-亚氨基苯并噻唑啉和 1-烷基-4-甲基喹啉鎓盐缩合制备不对称单次甲基花青的改进方法。该制备方法被认为比涉及2-甲硫基-3-甲基苯并噻唑鎓盐和1-烷基-4-甲基喹啉鎓盐缩合的更常用方法更环保。合成产生重要的荧光嵌入染料 1-甲基-4-[(3-甲基苯并噻唑啉-2-亚甲基)次甲基]-喹啉鎓盐，或其苯并噻唑核 6 位上有取代基的衍生物。起始材料是可商购的或者可以容易地合成。这些生物非共价标记的制备过程简单可靠。
An improved method for the preparation of asymmetric monomethine cyanines by the condensation of 6-substituted-2-iminobenzothiazolines and 1-alkyl-4-methylquinolinium salts is described. The preparation can be considered as being more environmentally friendly than the more usual method involving condensation of 2-methylthio-3-methylbenzothiazolium salts and 1-alkyl-4-methylquinolinium salts. The synthesis results in the important fluorogenic intercalating dye 1-methyl-4-[(3-methylbenzothiazoline-2-ylidene)methine]-quinolinium salts, or its derivatives with substituents at the 6-position in the benzothiazole nucleus. The starting materials are commercially available or can be easily synthesized. The procedure for the preparation of these biological non-covalent labels is simple and reliable.