DNA hole transport on an electrode: Application to effective photoelectrochemical SNP typing
DNA hole transport on an electrode: Application to effective photoelectrochemical SNP typing
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DOI：
10.1021/ja057040t
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发表时间：
2006-01-18
影响因子：
15
通讯作者：
Saito, I
中科院分区：
文献类型：
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作者：
Okamoto, A;Kamei, T;Saito, I
DNA的一个有用的特性是通过DNA的远距离空穴传输很容易实现。光刺激下通过DNA的远程空穴传输在开发一种概念上新的电化学单核苷酸多态性（SNP）分型方法方面具有前景，可作为基因诊断和药物遗传学的通用平台。我们已经将设计用于光刺激远程空穴传输的人工DNA应用于SNP分型。通过将装有光敏剂的DNA探针固定在金工作电极上，与含有SNP位点的目标DNA链杂交，我们观察到阴极光电流，该电流根据特定位点的碱基性质而显着变化。使用空穴传输碱基的组合构成了一种非常强大的单步电化学分析方法，适用于所有类型序列的SNP分型。
A useful feature of DNA is that long-range hole transport through DNA is readily achieved. Photostimulated long-range hole transport through DNA has prospective use in the development of a conceptually new electrochemical single-nucleotide polymorphism (SNP) typing method for use as a versatile platform for gene diagnostics and pharmacogenetics. We have applied artificial DNAs designed for photostimulated long-range hole transport through DNA to SNP typing. By hybridizing photosensitizer-equipped DNA probes, immobilized on gold working electrodes, with a target DNA strand containing an SNP site, we observed a cathodic photocurrent, which markedly changed depending on the nature of the base at the specific site. The use of a combination of hole-transporting bases constitutes a very powerful method for a single-step electrochemical assay applicable to SNP typing of all types of sequences.