Expression of human lactoferrin in milk of transgenic mice
Expression of human lactoferrin in milk of transgenic mice
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DOI：
10.1007/bf01974087
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发表时间：
1994-03
影响因子：
3
通讯作者：
G. Platenburg;E. Kootwijk;P. Kooiman;S. L. Woloshuk;J. Nuijens;P. Krimpenfort;F. Pieper;H. A. Boer-H.-A.-Bo
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文献类型：
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作者：
G. Platenburg;E. Kootwijk;P. Kooiman;S. L. Woloshuk;J. Nuijens;P. Krimpenfort;F. Pieper;H. A. Boer-H.-A.-Bo
描述了人乳铁蛋白（hLF）在转基因小鼠乳汁中的表达。将牛αS1-酪蛋白基因的调控序列与hLF cDNA的编码序列融合，获得了几种转基因小鼠。人LF RNA仅在哺乳期女性的乳腺中检测到，并且仅在哺乳期开始后检测到。使用北方印迹和引物延伸分析未检测到异常RNA产物。母乳中的hLF浓度范围为小于0.1至36 μg ml−1。因此，表达的人LF与人乳来源的LF在分子量和与单克隆和多克隆抗体的免疫反应性方面没有差异。
The expression of human lactoferrin (hLF) in the milk of transgenic mice is described. Regulatory sequences derived from the bovine αS1-casein gene were fused to the coding sequence of the hLF cDNA and several lines of transgenic mice were generated. Human LF RNA was detected exclusively in the mammary gland of lactating females and only after the onset of lactation. No aberrant RNA products could be detected using northern blotting and primer extension analysis. The hLF concentrations in the milk ranged from less than 0.1 to 36 μg ml−1. Human LF thus expressed did not differ from human milk derived LF, with respect to molecular mass and immunoreactivity with monoclonal and polyclonal antibodies.