Serum is the preferred clinical specimen for diagnosis of human brucellosis by PCR
Serum is the preferred clinical specimen for diagnosis of human brucellosis by PCR
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DOI：
10.1128/jcm.39.4.1661-1664.2001
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发表时间：
2001-04-01
影响因子：
9.4
通讯作者：
Legakis, NJ
中科院分区：
文献类型：
--
作者：
Zerva, L;Bourantas, K;Legakis, NJ
人类布鲁氏菌病在许多发展中国家造成严重的公共卫生问题，需要快速和准确的诊断。建立了扩增31 kDa流产布鲁氏菌抗原蛋白基因部分序列的聚合酶链式反应方法，并对31例布鲁氏菌病患者和45例健康人的全血和血清标本进行了检测。除1例患者外，其余患者全血或血清中均可检出布鲁氏菌DNA(联合敏感度为97%)，但血清标本的敏感度(94%)高于全血(61%)。该方法具有良好的特异性(100%)。还建立了针对布鲁氏菌另一个基因区域(OMP-2)的确认性PCR方法，但缺乏敏感性。血清是用聚合酶链式反应诊断布鲁氏菌病的最佳标本，这一选择简化了检测并缩短了周转时间。
Human brucellosis poses a significant public health problem in many developing countries and requires fast and accurate diagnosis. A PCR assay amplifying part of the 31-kDa Brucella abortus antigenic protein gene sequence was developed and applied to whole-blood and serum samples from 31 brucellosis patients and 45 healthy individuals. All patients except one had detectable Brucella DNA in either whole blood or serum (combined sensitivity, 97%), but, the assay sensitivity was higher with serum samples (94%) than with whole-blood samples (61%). The assay specificity was excellent (100%). A confirmatory PCR assay targeting another Brucella gene region (omp-2) was also developed but lacked sensitivity. Serum is the optimal specimen for the diagnosis of brucellosis by PCR, a choice that leads to assay simplification and shortens turnaround time.