A reduction-sensitive carrier system using mesoporous silica nanospheres with biodegradable polyester as caps
A reduction-sensitive carrier system using mesoporous silica nanospheres with biodegradable polyester as caps
复制标题
使用介孔二氧化硅纳米球和可生物降解聚酯作为帽的还原敏感载体系统
DOI：
10.1039/c3cp51947c
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发表时间：
2013-01-01
影响因子：
3.3
通讯作者：
Huang, Yubin
中科院分区：
文献类型：
--
作者：
He, Hongyan;Kuang, Huihui;Huang, Yubin
介孔二氧化硅纳米颗粒（MSN）-聚合物杂化物结合脂肪族生物降解聚酯帽的表面上首先开发，以操纵抗癌药物的智能细胞内释放。首先，聚（乙二醇）-b-聚（ε-己内酯）（PEG-PCL）通过二硫键成功地接枝到MSN的表面上，在肿瘤细胞的还原环境下可以切割。抗癌药物多柔比星（DOX）被封装到颗粒孔中。体外药物释放曲线显示，DOX释放在pH 7.4时受到聚合物帽的显著限制，而GSH的加入大大加速了DOX释放。细胞毒性评价显示与杂化颗粒具有良好的生物相容性。激光共聚焦显微镜观察到细胞内快速的内吞和DOX的释放。DOX负载的颗粒表现出与游离DOX相当的对HeLa细胞的抗肿瘤活性，并且表现出时间依赖性。这项工作开发了一种广泛的方法，利用脂肪族可生物降解聚酯作为聚合物帽MSN控制药物输送。这篇论文可能为癌症治疗提供一个潜在的选择。
Mesoporous silica nanoparticles (MSN)-polymer hybrid combined with the aliphatic biodegradable polyester caps on the surface were first developed in order to manipulate the smart intracellular release of anticancer drugs. First, poly(ethylene glycol)-b-poly(epsilon-caprolactone) (PEG-PCL) was successfully grafted on the surface of MSN via disulfide bonds which could cleave under a reduction environment in tumor cells. The anticancer drug doxorubicin (DOX) was encapsulated into the particle pores. The in vitro drug release profile showed that DOX release was significantly restricted by the polymer caps at pH 7.4, while it was greatly accelerated upon the addition of GSH. Cytotoxicity evaluation showed good biocompatibility with the hybrid particles. Fast endocytosis and intracellular DOX release were observed by confocal laser scanning microscopy (CLSM). The DOX-loaded particles exhibited comparable antitumor activity with free DOX towards HeLa cells and showed in a time-dependent manner. This work developed an extensive method of utilizing aliphatic biodegradable polyesters as polymer caps for MSN to control drug delivery. The paper might offer a potential option for cancer therapy.