Superficial zone protein affects boundary lubrication on the surface of mandibular condylar cartilage
Superficial zone protein affects boundary lubrication on the surface of mandibular condylar cartilage
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DOI：
10.1007/s00441-011-1156-z
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发表时间：
2011-05-01
影响因子：
3.6
通讯作者：
Tanne, Kazuo
中科院分区：
文献类型：
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作者：
Tanimoto, Kotaro;Kamiya, Takashi;Tanne, Kazuo
本实验观察了髁突软骨表面表浅带蛋白（SZP）的定位及其边界润滑作用。从6 ~ 9月龄雌性猪下颌髁突软骨表层分离软骨细胞。对培养的软骨细胞施加7%或21%细胞伸长的循环拉伸应变。软骨基质蛋白和分泌型磷脂酶A（2）（sPLA（2））的基因表达水平通过实时聚合酶链反应分析进行定量。在使用0.1 U/ml sPLA（2）处理前后，通过摩擦测试仪测量下颌髁突表面的摩擦系数。SZP和I型胶原的mRNA水平显着较高的软骨细胞从表面层比在其他层。细胞伸长7%和21%的循环张应变使SZP mRNA水平上调。21%细胞伸长的周期性拉伸应变上调sPLA（2）mRNA水平。经sPLA处理后，髁突表面的摩擦系数显著增加（2）。用sPLA去除下颌髁突软骨表层的SZP（2）导致关节软骨表面的摩擦系数显著增加。
We examined the localization and boundary lubricating function of superficial zone protein (SZP) on the surface of mandibular condylar cartilage. Chondrocytes were separated from the surface layer of mandibular condylar cartilage of 6- to 9-month-old female pigs. A cyclic tensile strain of 7% or 21% cell elongation was applied to the cultured chondrocytes. Gene expression levels of cartilage matrix proteins and secretory phospholipase A(2) (sPLA(2)) were quantified by real-time polymerase chain reaction analysis. The friction coefficient of the mandibular condylar surface was measured by a friction tester before and after treatment with 0.1 U/ml sPLA(2). Significantly higher mRNA levels of SZP and type I collagen were found in chondrocytes from the superficial layer than in those in the other layers. The SZP mRNA level was up-regulated by cyclic tensile strain of 7% and 21% cell elongation. Cyclic tensile strain of 21% cell elongation up-regulated the sPLA(2) mRNA level. The friction coefficient of the condylar surface was increased significantly by treatment with sPLA(2). The removal of SZP from the surface layer of mandibular condylar cartilage by sPLA(2) resulted in a significant increase in the friction coefficient on the surface of articular cartilage.