Curcumin improves growth factors expression of bovine cumulus-oocyte complexes cultured in peritoneal fluid of women with endometriosis
Curcumin improves growth factors expression of bovine cumulus-oocyte complexes cultured in peritoneal fluid of women with endometriosis
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DOI：
10.18502/ijrm.v16i12.3683
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发表时间：
2018-12-01
影响因子：
1.3
通讯作者：
Widjiati, Widjiati
中科院分区：
文献类型：
--
作者：
Hendarto, Hendy;Widyanugraha, Muhammad Yohanes Ardianta;Widjiati, Widjiati
背景资料：子宫内膜异位症不孕妇女的腹腔液（PF）中含有可能干扰卵泡生成的炎症介质。目的：目的是通过评估生长分化因子-9（GDF-9）、Kit配体（KitL）、和肿瘤坏死因子α（TNF α）在与患有子宫内膜异位症的不孕妇女的PF一起培养的牛卵丘-卵母细胞复合体（COC）中的表达。材料和方法：在这项实验研究中，2015年1月至7月期间转诊到Dr Sutomo Hospital的21名不孕女性（年龄在20至40岁之间）入组。从牛卵巢有腔卵泡中吸出COCs。PF收集不孕妇女子宫内膜异位症行腹腔镜检查不孕症的评估。姜黄素是一种强抗炎的姜黄，加入到组织培养基199（TCM 199）和PF培养基中。将牛COCs培养于3组培养基中：1. TCM 199，2. TCM 199 + PF，和3. TCM 199 + PF +姜黄素。结果：在PF中加入姜黄素培养的牛COCs中GDF-9的表达（2.67 ± 0.98）比不加姜黄素培养的牛COCs中GDF-9的表达（0.50 ± 0.67）增加（p
Background: Peritoneal fluid (PF) from infertile women with endometriosis contains inflammatory mediators that may interfere with folliculogenesis.Objective: The aim was to evaluate the effects of curcumin on growth factors expression by evaluating Growth Differentiation Factor-9 (GDF-9), Kit Ligand (KitL), and Tumor Necrosis Factor alpha (TNF alpha) expressions in bovine cumulus-oocyte complexes (COC)s cultured with PF from infertile women with endometriosis.Materials and Methods: In this experimental study, 21 infertile women (aged between 20 and 40 years) who refered to Dr Sutomo Hospital from January to July 2015 were enrolled. COCs were aspirated from antral follicles of bovine ovaries. PF was collected from infertile women with endometriosis undergoing laparoscopy for infertility evaluation. Curcumin, a strong anti-inflammatory turmeric, was added in Tissue Culture Medium 199 (TCM199) and PF for culture medium. Bovine COCs were cultured into three groups of the medium: 1. TCM199, 2. TCM199 + PF, and 3. TCM199 + PF + curcumin. GDF9, KitL, and TNF alpha expressions were examined using immunohistochemistry technique.Results: GDF-9 expression of bovine COCs cultured in PF with curcumin addition (2.67 +/- 0.98) was found to increase compared to those cultured without curcumin (0.50 +/- 0.67) (p