Bacteria from the citrus phylloplane can disrupt cell-cell signalling in Xanthomonas citri and reduce citrus canker disease severity
Bacteria from the citrus phylloplane can disrupt cell-cell signalling in Xanthomonas citri and reduce citrus canker disease severity
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DOI：
10.1111/ppa.12466
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发表时间：
2016-06-01
期刊：
影响因子：
2.7
通讯作者：
Ferro, J. A.
中科院分区：
文献类型：
--
作者：
Caicedo, J. C.;Villamizar, S.;Ferro, J. A.
柑橘黄单胞菌柑橘溃疡病是一种几乎影响所有类型柑橘作物的病害，柑橘溃疡病是柑橘溃疡病的病原体。特定Xcc致病因子的产生由基因簇rpf控制，该基因簇rpf编码被称为群体感应（QS）的细胞-细胞通信系统的元件，该元件由扩散信号因子（DSF）家族的分子介导。通过信号降解或过度产生干扰细胞-细胞信号传导，也称为群体淬灭，已被建议作为控制细菌疾病的策略。在这项研究中，从柑橘叶片中分离出三种细菌菌株，它们具有破坏Xcc中QS信号传导的能力。对甜橙子的致病性测定表明，假单胞菌属和芽孢杆菌属的细菌也具有较强的减轻柑橘溃疡病严重程度的能力。这些影响与细菌附着和生物膜形成的改变有关，这些因素已知有助于Xcc毒力。这些群体淬灭细菌可能是生物防治过程中非常有价值的工具，并为目前用于治疗柑橘溃疡病的传统铜治疗提供了替代方案。
Xanthomonas citri subsp. citri (Xcc) is the causal agent of citrus canker, a disease that affects almost all types of citrus crops. Production of particular Xcc pathogenicity factors is controlled by a gene cluster rpf, which encodes elements of a cell-cell communication system called quorum sensing (QS), mediated by molecules of the diffusible signal factor (DSF) family. Interference with cell-cell signalling, also termed quorum quenching, either by signal degradation or over-production, has been suggested as a strategy to control bacterial disease. In this study, three bacterial strains were isolated from citrus leaves that displayed the ability to disrupt QS signalling in Xcc. Pathogenicity assays in sweet orange (Citrus sinensis) showed that bacteria of the genera Pseudomonas and Bacillus also have a strong ability to reduce the severity of citrus canker disease. These effects were associated with alteration in bacterial attachment and biofilm formation, factors that are known to contribute to Xcc virulence. These quorum-quenching bacteria may represent a highly valuable tool in the process of biological control and offer an alternative to the traditional copper treatment currently used to treat citrus canker disease.