Organelle isolation: functional mitochondria from mouse liver, muscle and cultured filroblasts
Organelle isolation: functional mitochondria from mouse liver, muscle and cultured filroblasts
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DOI：
10.1038/nprot.2006.478
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Scorrano, Luca
中科院分区：
文献类型：
--
作者：
Frezza, Christian;Cipolat, Sara;Scorrano, Luca
线粒体参与细胞的关键代谢反应，并调节包括细胞凋亡在内的重要信号通路。尽管有几种原位研究线粒体功能的方法，但研究从不同组织和培养细胞中分离出来的有功能的线粒体仍具有更多无可比拟的优势。该实验方案阐述了从培养细胞、肝脏和肌肉中获取高产量有功能线粒体的逐步操作过程。此处描述的分离过程需要1 - 2小时，具体时间取决于细胞器的来源。极谱分析可在1小时内完成。
Mitochondria participate in key metabolic reactions of the cell and regulate crucial signaling pathways including apoptosis. Although several approaches are available to study mitochondrial function in situ are available, investigating functional mitochondria that have been isolated from different tissues and from cultured cells offers still more unmatched advantages. This protocol illustrates a step-by-step procedure to obtain functional mitochondria with high yield from cells grown in culture, liver and muscle. The isolation procedures described here require 1 - 2 hours, depending on the source of the organelles. The polarographic analysis can be completed in 1 hour.