Differential transplantability of tumor-associated stromal cells
Differential transplantability of tumor-associated stromal cells
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DOI：
10.1158/0008-5472.can-04-1268
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发表时间：
2004-09-01
期刊：
影响因子：
11.2
通讯作者：
Jain, RK
中科院分区：
文献类型：
--
作者：
Duda, DG;Fukumura, D;Jain, RK
移植时，肿瘤碎片中含有“客体”细胞：来自原始宿主的内皮细胞和其他基质细胞。在这里，我们研究了基因标记的内皮和非内皮基质细胞在同基因小鼠体内移植后的命运。我们报告，与肿瘤或脂肪组织相关的血管生成基质在移植后持续存在，保持功能，并在植入后4周以上控制移植组织片段的初始新生血管形成。令人惊讶的是，客体内皮细胞比其他基质细胞存活时间更长，后者在3周后被宿主激活的成纤维细胞取代。肿瘤基质的可移植性表明，决定其生存能力的肿瘤异种移植血管形成潜力取决于异种移植体内客体内皮细胞和其他基质细胞的存在。这些肿瘤组织移植的研究为探索上皮-间质相互作用在肿瘤异质性和耐药性研究中的作用提供了平台。
At the time of transplantation, tumor fragments contain "passenger" cells: endothelial cells and other stromal cells from the original host. Here, we investigated the fate of genetically labeled endothelial and nonendothelial stromal cells after transplantation in syngeneic mice. We report that angiogenic stroma associated with tumor or adipose tissue persists when transplanted, remains functional, and governs the initial neovascudarization of grafted tissue fragments for more than 4 weeks after implantation. Surprisingly, the passenger endothelial cells survive longer than other stromal cells, which are replaced by host-activated fibroblasts after 3 weeks. The transplantability of tumor stroma suggests that the anglogenic potential of a tumor xenograft, which determines its viability, depends on the presence of passenger endothelial cells and other stromal cells within the xenograft. These studies of tumor tissue transplantation provide a platform for exploring the role of epithelial-stromal interactions in studies of tumor heterogeneity and drug resistance.