ANALYSIS OF THE IMMUNOHISTOCHEMICAL LOCALIZATION OF COLLAGEN TYPE-III AND TYPE-V FOR THE TIME-ESTIMATION OF HUMAN SKIN WOUNDS
ANALYSIS OF THE IMMUNOHISTOCHEMICAL LOCALIZATION OF COLLAGEN TYPE-III AND TYPE-V FOR THE TIME-ESTIMATION OF HUMAN SKIN WOUNDS
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DOI：
10.1007/bf01222117
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发表时间：
1993-05-01
影响因子：
2.1
通讯作者：
EISENMENGER, W
中科院分区：
文献类型：
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作者：
BETZ, P;NERLICH, A;EISENMENGER, W
在79例手术治疗的人皮肤伤口中，用免疫化学方法观察III型和V型胶原，伤口年龄在8小时和2.5个月之间。网状结构阳性染色的III型胶原蛋白和相关的成纤维细胞在伤口区域首次检测到在2.5天的皮肤病变，经常发生在伤口超过5天。约3天后，V型胶原首先出现在伤口区域，稍晚于III型胶原，并且在存活时间为6天或更长的伤口中定期检测到。因此，III型或V型胶原蛋白的免疫组织化学检测表明伤口年龄至少为2 - 3天。在足够数量的样本中缺乏阳性反应表明伤口年龄小于6天。尽管在较旧的伤口（伤口年龄2.5个月）中也可以检测到两种类型的胶原蛋白，但由于观察到可以观察到修复过程的时间段取决于伤口面积的范围，因此无法提供较旧皮肤伤口时间估计的进一步信息。
Collagen type III and V were visualized immunohistochemically in 79 surgically treated human skin wounds with a wound age between 8 h and 2.5 months. Network-like structures positively staining for collagen type III and associated with fibroblastic cells in the wound area were first detectable in a 2.5-day-old skin lesion and occurred regularly in wounds more than 5 days old. Collagen type V appeared first in the wound area after about 3 days, slightly later than collagen type III, and was detectable regularly in wounds with a survival time of 6 days or more. The immunohistochemical detection of collagen type III or type V thus indicates a wound age of at least 2-3 days. The lack of a positive reaction in a sufficient number of specimens indicates a wound age of less than 6 days. Even though both collagen types could also be detected in older wounds (wound age 2.5 months), further information for the time-estimation of older skin wounds cannot be given due to the observation that the time period during which reparative processes can be observed depends on the extent of the wound area.