Control of auxin-induced callus formation by bZIP59-LBD complex in Arabidopsis regeneration
Control of auxin-induced callus formation by bZIP59-LBD complex in Arabidopsis regeneration
复制标题
bZIP59-LBD 复合物在拟南芥再生中控制生长素诱导的愈伤组织形成
DOI：
10.1038/s41477-017-0095-4
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发表时间：
2018-02-01
期刊：
影响因子：
18
通讯作者：
Hu, Yuxin
中科院分区：
文献类型：
--
作者：
Xu, Chongyi;Cao, Huifen;Hu, Yuxin
生长素诱导多能愈伤组织是植物离体再生所需的一种典型的细胞命运变化。然而，生长素诱导的愈伤组织形成的分子调控在很大程度上是难以捉摸的。我们之前发现了四个拟南芥生长素诱导的控制愈伤组织形成的侧器官边界域（LBD）转录因子。在这里，我们报道了拟南芥基本区/亮氨酸拉链motif 59 （AtbZIP59）转录因子与lbd形成复合物，指导生长素诱导的愈伤组织形成。我们发现生长素稳定了AtbZIP59并增强了它与LBD的相互作用，AtbZIP59的破坏抑制了生长素诱导的愈伤组织的形成，而AtbZIP59的过表达则触发了自主愈伤组织的形成。AtbZIP59-LBD16直接靶向FAD-binding Berberine （FAD-BD）基因并促进其转录，从而促进愈伤组织的形成。这些发现将AtbZIP59-LBD复合物定义为生长素诱导的愈伤组织形成过程中细胞命运变化的关键调节因子，这为植物再生和可能的发育程序的分子调控提供了新的见解。
Induction of pluripotent cells termed callus by auxin represents a typical cell fate change required for plant in vitro regeneration; however, the molecular control of auxin-induced callus formation is largely elusive. We previously identified four Arabidopsis auxin-inducible Lateral Organ Boundaries Domain (LBD) transcription factors that govern callus formation. Here, we report that Arabidopsis basic region/leucine zipper motif 59 (AtbZIP59) transcription factor forms complexes with LBDs to direct auxin-induced callus formation. We show that auxin stabilizes AtbZIP59 and enhances its interaction with LBD, and that disruption of AtbZIP59 dampens auxin-induced callus formation whereas overexpression of AtbZIP59 triggers autonomous callus formation. AtbZIP59-LBD16 directly targets a FAD-binding Berberine (FAD-BD) gene and promotes its transcription, which contributes to callus formation. These findings define the AtbZIP59-LBD complex as a critical regulator of auxin-induced cell fate change during callus formation, which provides a new insight into the molecular regulation of plant regeneration and possible developmental programs.