Studies on human thyroxine-binding globulin. 8. Isoelectric focusing evidence for microheterogeneity of thyroxine-binding globulin.
Studies on human thyroxine-binding globulin. 8. Isoelectric focusing evidence for microheterogeneity of thyroxine-binding globulin.
复制标题
人甲状腺素结合球蛋白的研究。
DOI：
10.1016/0003-9861(73)90294-4
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发表时间：
1973
影响因子：
3.9
通讯作者：
S. Williams
中科院分区：
文献类型：
--
作者：
J. S. Marshall;J. Pensky;S. Williams
高度纯化的甲状腺素结合球蛋白从合并的人血清均匀的常规标准，进行等电聚焦在聚丙烯酰胺凝胶中的pH梯度从3-6，产生了至少9个蛋白带的模式。所有这些条带似乎都与甲状腺素结合。完全去唾液酸化的甲状腺素结合球蛋白进行等电聚焦产生相同数量和模式的带位于不同的区域中的pH梯度。从单个供体的血清中纯化的甲状腺素结合球蛋白进行等电聚焦。该甲状腺素结合球蛋白具有相同的蛋白质条带模式，但合并血清中甲状腺素结合球蛋白中观察到的一条主要条带不存在。这些现象的几种可能的解释进行了讨论。
Highly purified thyroxine-binding globulin from pooled human serum homogeneous by conventional criteria, subjected to isoelectric focusing in polyacrylamide gels in a pH gradient from 3–6, produced a pattern of at least nine stainable protein bands. All of these bands appeared to bind thyroxine. Completely desialylated thyroxine-binding globulin subjected to isoelectric focusing produced the same number and pattern of bands located at a different area in the pH gradient. Thyroxine-binding globulin purified from the serum of a single donor was subjected to isoelectric focusing. This thyroxine-binding globulin had the same pattern of protein bands with the exception that one of the major bands seen in the thyroxine-binding globulin from pooled serum was absent. Several possible explanations for these phenomena are discussed.