Fructooligosaccharide associated with celecoxib reduces the number of aberrant crypt foci in the colon of rats
Fructooligosaccharide associated with celecoxib reduces the number of aberrant crypt foci in the colon of rats
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DOI：
10.1051/rnd:2003028
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发表时间：
2003-07-01
期刊：
影响因子：
--
通讯作者：
Blottière, HM
中科院分区：
文献类型：
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作者：
Buecher, B;Thouminot, C;Blottière, HM
根据Burkitt的假设，膳食纤维可能会预防结直肠癌的发展。在老鼠身上，研究表明只有产生丁酸的纤维具有保护作用。同时，在人类中，针对环氧合酶的非类固醇抗炎药物已被证明对结直肠癌具有保护作用。其中，环氧合酶-2选择性抑制剂比非选择性药物副作用小，有望作为化学预防药物。我们的目的是分析丁酸产生纤维和COX-2抑制剂之间的联系在大鼠异常隐窝病灶(ACF)发展中的作用。Fisher F344大鼠饲喂(1)标准低纤维对照饲料；(2)添加1500ppm塞来昔布的标准饲料；(3)添加6%低聚果糖(FOS)的饲料；(4)同时添加塞来昔布和低聚果糖的饲料。3周后，两次注射偶氮甲烷，15周后测定ACF数量。对照组ACF为43.8+/-6.4。在饮食中单独添加低聚果糖或塞来昔布并没有显著改变这一数字。然而，FOS和塞来昔布的联合应用导致ACF的数量减少了61%(P<；0.01)。每个病灶的异常隐窝数目也减少了。因此，尽管塞来昔布或低聚果糖单独作用不明显，但丁酸产生纤维和塞来昔布的联合作用在预防ACF的发展方面是有效的。这项初步研究认为，这种联系具有很强的保护作用，值得进一步研究。
According to Burkitt's hypothesis, dietary fibres may protect against the development of colorectal cancer. In rats, studies have shown that only butyrate-producing fibres are protective. In parallel, in humans, non-steroidal anti-inflammatory drugs, which target cyclooxygenases, have been shown to display a protective effect against colorectal cancer. Among them, COX-2-selective inhibitors which present less side effects than non-selective agents, are promising as chemopreventive agents. Our aim was to analyse the effect of an association between butyrate-producing fibres and the COX-2 inhibitor on the development of aberrant crypt foci (ACF) in rats. Fisher F344 rats were fed with ( 1) a standard low fibre control diet; ( 2) the standard diet supplemented with 1500 ppm celecoxib; ( 3) a diet supplemented with 6% fructo-oligosaccharide (FOS); and ( 4) a diet with both celecoxib and FOS. Three weeks later, the rats were injected twice with azoxymethane and the number of ACF was determined 15 weeks later. In the control group, 43.8 +/- 6.4 ACF were found. This number was not significantly modified by the addition of FOS or celecoxib alone to the diet. However, the association of FOS and celecoxib resulted in a 61% reduction in the number of ACF (P < 0.01). The number of aberrant crypt per foci was also reduced. Thus, although no significant effect of celecoxib or FOS alone was identified, the association of butyrate-producing fibre and celecoxib was effective in preventing the development of ACF. This preliminary study argues for a strong protective effect of such an association which deserves further studies.