Repression of the steroidogenic acute regulatory gene by the multifunctional transcription factor Yin Yang 1.
Repression of the steroidogenic acute regulatory gene by the multifunctional transcription factor Yin Yang 1.
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DOI：
10.1210/endo.143.3.8668
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发表时间：
2002-03
期刊：
影响因子：
4.8
通讯作者：
A. Nackley;W. Shea-Eaton;D. Lopez;M. McLean
中科院分区：
文献类型：
--
作者：
A. Nackley;W. Shea-Eaton;D. Lopez;M. McLean
The transport of cholesterol to the inner mitochondrial membrane by the steroidogenic acute regulatory (StAR) protein is a critical step in steroidogenesis. The current study was designed to examine whether the multifunctional transcription factor Yin Yang 1 (YY1) was able to repress activation of the StAR gene. YY1 bound to three putative YY1-binding sites in the rat StAR promoter. Cotransfection of the StAR promoter linked to a luciferase reporter gene and YY1 in the presence or absence of sterol regulatory element binding protein-1a (SREBP-1a) resulted in transcriptional repression. YY1 was found to colocalize in the nucleus with SREPB-1a. YY1 inhibited SREBP-1a/nuclear factor Y (NF-Y) enhancement of StAR activation and YY1 decreased SREBP-1a binding to a sterol regulatory element in the presence or absence of NF-Y. YY1 also decreased NF-Y binding to a nonconsensus NF-Y-binding motif in the StAR promoter. These studies provide novel information on the mechanisms of YY1-mediated repression of the StAR gene.
