Identification of Ty1-copia retrotransposons in three ectomycorrhizal basidiomycetes:: evolutionary relationships and use as molecular markers
Identification of Ty1-copia retrotransposons in three ectomycorrhizal basidiomycetes:: evolutionary relationships and use as molecular markers
复制标题
DOI：
10.1007/s00294-002-0363-2
复制
发表时间：
2003-04-01
期刊：
影响因子：
2.5
通讯作者：
Tagu, D
中科院分区：
文献类型：
--
作者：
Díez, J;Béguiristain, T;Tagu, D
我们通过PCR扩增和测序的46个部分Ty 1-copia逆转录酶（RT）序列从外生菌根担子菌豆和Laccaria bicolor和宿主树蓝桉。系统发育分析表明，这些序列代表一类新的Ty 1-copia RT，特征的担子菌，但有关植物Ty 1-copia反转录转座子。建立了L.双色菌株，设计与RT退火的外向PCR引物。该方法是反转录转座子间扩增多态性（IRAP）分析的改进，能够检测基因组中Ty 1-copia元件插入位点内的多态性或变化。利用这种方法，我们研究了Ty 1-copia元件的转座是否与在L. bicolor S238，一种用于法国花旗松种植园的接种菌株。结果表明，在L.双色S238。我们在这里首次报道了担子菌中Ty 1-copia逆转录转座子序列的存在，这为开发新的分子标记提供了合适的靶点。
We amplified by PCR and sequenced 46 partial Ty1-copia reverse transcriptase (RT) sequences from the ectomycorrhizal basidiomycetes Pisolithus and Laccaria bicolor and the host tree Eucalyptus globulus. Phylogenetic analyses indicated that these sequences represent a new class of Ty1-copia RT, characteristic of basidiomycetes but related to plant Ty1-copia retrotransposons. To generate fingerprints of L. bicolor strains, outward facing PCR primers annealing to RTs were designed. This method, which is a modification of the inter-retrotransposon amplified polymorphism (IRAP) analysis, enables the detection of polymorphisms or changes within the insertion sites of Ty1-copia elements in the genome. Using this method, we investigated whether the transposition of Ty1-copia elements was related to the somaclonal variation observed in L. bicolor S238, an inoculant strain used in French Douglas-fir plantations. Data indicated that no differences in the IRAP fingerprints were detected in phenotypic variants of L. bicolor S238. We reported here for the first time the presence of Ty1-copia retrotransposon sequences in basidiomycetes, which resulted in suitable targets for developing new molecular markers.