Novel analytical approach to find distinctive odor compounds from garlic cloves infested by the potato-rot nematode Ditylenchus destructor using gas chromatography–olfactometry (GC–O) with heart-cut enrichment system
Novel analytical approach to find distinctive odor compounds from garlic cloves infested by the potato-rot nematode Ditylenchus destructor using gas chromatography–olfactometry (GC–O) with heart-cut enrichment system
复制标题
使用气相色谱-嗅觉测定法 (GC-O) 和中心切割富集系统，从被马铃薯腐烂线虫腐烂茎线虫侵染的蒜瓣中寻找独特气味化合物的新分析方法
DOI：
10.1007/s41348-020-00349-3
复制
发表时间：
2020
影响因子：
2
通讯作者：
T. Yoshiga
中科院分区：
文献类型：
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作者：
Misato Matsumoto;D. Ueno;Rie Aoyama;Katsuhisa Sato;Y. Koga;Taiki Higuchi;Hideaki Matsumoto;Kou Nishimuta;Shunsuke Haraguchi;Hideki Miyamoto;Tomokazu Haraguchi;T. Yoshiga
马铃薯腐线虫对日本大蒜生产造成严重危害。被线虫污染的大蒜种子瓣经常被用于种植，因为被线虫感染的大蒜瓣很难与健康的大蒜瓣区分开来。为了能够对线虫感染的大蒜进行无损检测，本研究建立了一种新的GC-O和GC-MS结合心脏切口富集技术的分析系统。本研究的目的是找到一种分析方法来识别来自受线虫感染的大蒜的主观可感知气味的存在，并评估这些新型分析系统的有效性。该系统检测了10种与线虫感染大蒜相关的气味活性，鉴定出“烯丙基甲基硫醚”和“2-甲基-1-丁醇”是主要的气味化合物（1-丙硫醇是候选化合物）。这一结果表明，本研究中引入的新型分析技术提供了足够的数据来鉴定侵染大蒜的气味化合物。两种不同气味的化合物可作为蒜瓣线虫感染的无损检测指标。
The potato-rot nematodeDitylenchus destructor(Tylenchida: Anguinidae) causes severe damage to garlic production in Japan. Nematode-contaminated garlic seed cloves are often used for planting, because nematode-infested cloves are difficult to discriminate from healthy ones. To be able to find nematode-infested garlic in a nondestructive way, here this study introduced a novel analytical system of GC–O and GC–MS with heart-cut enrichment technique. The objective of this research was to find an analytical approach to identify the existence of subjectively perceptible odors which derive from the garlic infested with the nematode and to evaluate the effectiveness of those novel analytical systems. Ten odor activities associated with nematode-infested garlic were detected by the system, and “allyl methyl sulfide” and “2-methyl-1-butanol” were identified as major odor compounds (1-propanethiol was candidate). This result suggests that the novel analytical technique introduced in this study delivered sufficient data to identify the odor compounds from infested garlics. Two distinctive odor compounds might be used as indicators to detect nematode-infested garlic cloves with nondestructive way.