HEPATOCYTE GROWTH-FACTOR INHIBITS CELL-PROLIFERATION IN-VIVO OF RAT HEPATOCELLULAR CARCINOMAS INDUCED BY DIETHYLNITROSAMINE
HEPATOCYTE GROWTH-FACTOR INHIBITS CELL-PROLIFERATION IN-VIVO OF RAT HEPATOCELLULAR CARCINOMAS INDUCED BY DIETHYLNITROSAMINE
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DOI：
10.1093/carcin/16.4.841
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发表时间：
1995-04-01
期刊：
影响因子：
4.7
通讯作者：
MICHALOPOULOS, GK
中科院分区：
文献类型：
--
作者：
LIU, ML;MARS, WM;MICHALOPOULOS, GK
肝细胞生长因子（HGF）除了对各种细胞类型具有促有丝分裂、运动发生和形态发生功能外，还抑制几种癌细胞系（包括癌和肉瘤）的有丝分裂。在这里，我们报告，肝细胞生长因子也是螨抑制大鼠肝肿瘤诱导的二乙基亚硝胺。通过采用[H-3]胸苷和5-溴-2 '-脱氧尿苷的双标记技术测定HGF输注前后的细胞增殖，我们确定连续输注20 μ g总HGF抑制肿瘤细胞增殖50%。非肿瘤区域的标记显示了相反的结果，即HGF治疗组大鼠的标记高于对照组大鼠。这些结果表明，肝细胞生长因子对正常和肿瘤肝细胞在体内的生长有不同的影响。这些发现可能有助于理解HGF在肝癌发生中的作用，并为控制肝细胞癌的生长提供了额外的模式。
In addition to its mitogenic, motogenic and morphogenic functions on various cell types, hepatocyte growth factor (HGF) also suppresses mitosis in several cancer cell lines, including carcinomas and sarcomas. Here we report that HGF is also mite-inhibitory in rat liver tumors induced by diethylnitrosamine. By using a double labeling technique employing [H-3]thymidine and 5-bromo-2'-deoxyuridine to determine cell proliferation before and after HGF infusion, we determined that continuous infusion of 20 mu g total HGF inhibited tumor cell proliferation by 50%. The labeling in non-tumor areas showed the reverse result in that the labeling was higher in HGF-treated rats than control rats. These results indicate that HGF has different effects on growth of normal and tumorous hepatocytes in vivo. These findings may be of relevance in understanding the role of HGF in hepatocarcinogenesis and provide added modalities for controlling growth of hepatocellular carcinomas.