Glut1 promotes cell proliferation, migration and invasion by regulating epidermal growth factor receptor and integrin signaling in triple-negative breast cancer cells.
Glut1 promotes cell proliferation, migration and invasion by regulating epidermal growth factor receptor and integrin signaling in triple-negative breast cancer cells.
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DOI：
10.5483/bmbrep.2017.50.3.189
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发表时间：
2017-03
期刊：
影响因子：
3.8
通讯作者：
Shin I
中科院分区：
文献类型：
--
作者：
Oh S;Kim H;Nam K;Shin I
癌细胞中葡萄糖水平升高可归因于葡萄糖转运蛋白（GLUT）水平的增加。在人类恶性肿瘤细胞中，Glut1的表达增加。为了研究Glut1在乳腺癌中的其他作用，我们利用短发夹RNA（shRNA）系统在三阴性乳腺癌细胞系中沉默了Glut1。通过蛋白质印迹法和实时定量逆转录聚合酶链反应（qRT - PCR）验证了Glut1沉默。Glut1的敲低通过调节表皮生长因子受体/丝裂原活化蛋白激酶（EGFR/MAPK）信号通路以及整合素β1/原癌基因酪氨酸蛋白激酶/黏着斑激酶（integrin β1/Src/FAK）信号通路，导致细胞增殖、葡萄糖摄取、迁移和侵袭能力下降。这些结果表明，Glut1不仅作为葡萄糖转运蛋白发挥作用，而且在乳腺癌的肿瘤发生过程中还作为信号级联的调节因子。
Elevated glucose levels in cancer cells can be attributed to increased levels of glucose transporter (GLUT) proteins. Glut1 expression is increased in human malignant cells. To investigate alternative roles of Glut1 in breast cancer, we silenced Glut1 in triple-negative breast-cancer cell lines using a short hairpin RNA (shRNA) system. Glut1 silencing was verified by Western blotting and qRT-PCR. Knockdown of Glut1 resulted in decreased cell proliferation, glucose uptake, migration, and invasion through modulation of the EGFR/MAPK signaling pathway and integrin β1/Src/FAK signaling pathways. These results suggest that Glut1 not only plays a role as a glucose transporter, but also acts as a regulator of signaling cascades in the tumorigenesis of breast cancer.