Protein phylogeny of translation elongation factor EF-1 alpha suggests microsporidians are extremely ancient eukaryotes
Protein phylogeny of translation elongation factor EF-1 alpha suggests microsporidians are extremely ancient eukaryotes
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DOI：
10.1007/bf02198852
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发表时间：
1996-02-01
影响因子：
3.9
通讯作者：
Hasegawa, M
中科院分区：
文献类型：
--
作者：
Kamaishi, T;Hashimoto, T;Hasegawa, M
利用聚合酶链式反应(PCR)扩增了微孢子虫属无线粒体原生动物Glugea plecoglossi的翻译延长因子1α(EF-1α)的部分基因片段，并对其一级结构进行了分析。推测的氨基酸序列与真核生物典型的EF-1α高度不同，尽管当与三个主要王国的同源物比对时，它明显显示出真核特征。基于6种不同氨基酸替换随机模型的最大似然(ML)分析和最大简约(MP)分析一致地表明，在被分析的真核物种中，G.plecoglossi可能是真核生物最早的分支。微孢子虫可能是在线粒体共生发生之前就已经分化的极其古老的真核生物。
Partial regions of the mRNA encoding a major part of translation elongation factor 1 alpha (EF-1 alpha) from a mitochondrion-lacking protozoan, Glugea plecoglossi, that belongs to microsporidians, were amplified by polymerase chain reaction (PCR) and their primary structures were analyzed. The deduced amino acid sequence was highly divergent from typical EF-1 alpha's of eukaryotes, although it clearly showed a eukaryotic feature when aligned with homologs of the three primary kingdoms. Maximum likelihood (ML) analyses on the basis of six different stochastic models of amino acid substitutions and a maximum parsimony (MP) analysis consistently suggest that among eukaryotic species being analyzed, G. plecoglossi is likely to represent the earliest offshoot of eukaryotes. Microsporidians might be the extremely ancient eukaryotes which have diverged before an occurrence of mitochondrial symbiosis.