Characterization of two novel defense peptides from pea (Pisum sativum) seeds
Characterization of two novel defense peptides from pea (Pisum sativum) seeds
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DOI：
10.1006/abbi.2000.1824
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发表时间：
2000-06-15
影响因子：
3.9
通讯作者：
Kurtenbach, E
中科院分区：
文献类型：
--
作者：
Almeida, MS;Cabral, KMS;Kurtenbach, E
豌豆种子经硫酸铵分级分离、SephadexG-75凝胶过滤，得到一个具有抗尼日尔曲霉活性的组分，经反相高效液相色谱进一步纯化，得到两个富含半胱氨酸的小肽（Psd 1和Psd 2）。它们主要定位于豌豆荚的维管束和表皮组织中，并对几种真菌表现出高的抗真菌活性，显示IC 50值范围为0.04至22 μ g/ml。当A.尽管Psd 1和Psd 2的一级序列与其它植物防御素具有同源性，但它们不能容易地归属于任何已建立的组。(C)北京大学出版社.
A fraction that possesses antifungal activity against Aspergillus niger has been isolated from seeds of the pea (Pisum sativum) by ammonium sulfate fractionation followed by gel filtration on Sephadex G-75, On further purification by reverse-phase high performance liquid chromatography, two small cysteine-rich polypeptides mere obtained (Psd1 and Psd2). They are localized primarily in vascular bundles and epidermis tissues of pea pods and exhibit high antifungal activity toward several fungi, displaying IC50 values ranging from 0.04 to 22 mu g/ml. This inhibitory activity decreases when A. niger growth medium is supplemented with cations such as Ca2+, Mg2+, Na+, and K+, Although the primary sequence of both Psd1 and Psd2 shows homology with other plant defensins, they cannot easily be assigned to any established group. (C) 2000 Academic Press.