SYNERGISM OF CELLULASES FROM TRICHODERMA-REESEI IN THE DEGRADATION OF CELLULOSE
SYNERGISM OF CELLULASES FROM TRICHODERMA-REESEI IN THE DEGRADATION OF CELLULOSE
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DOI：
10.1038/nbt0885-722
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发表时间：
1985-01-01
期刊：
影响因子：
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通讯作者：
SCHULEIN, M
中科院分区：
文献类型：
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作者：
HENRISSAT, B;DRIGUEZ, H;SCHULEIN, M
从里氏木霉（Trichoderma reesei）中纯化的纤维二糖水解酶I和II（CBHI和CBHII）以及内切葡聚糖酶I和II（EGI和EGII）对不同底物的作用进行了评价。CBHI对大麦β-D-葡聚糖的降解具有典型的内模式。在纤维素底物中，CBHI和内切葡聚糖酶I或II之间的协同作用取决于底物的结构和超微结构特征。这种效果，无关的endo-exocotion，被发现与基板的中间结晶度，而弱或没有协同作用记录与纤维素微晶或可溶性羧甲基纤维素衍生物。还记录了CBHI和CBHII的混合物对纤维素的协同降解。另一方面，内切葡聚糖酶和CBHII之间的协同作用遵循预期的anendo-exocotion模式。这些结果为纤维素分解酶之间的多种类型的合作提供了支持证据。
The action of cellobiohydrolases I and II (CBHI and CBHII) and endoglucanases I and II (EGI and EGII) purified fromTri-choderma reeseiwas evaluated against various substrates. CBHI degraded the β-D-glucan from barley in a typicalendopattern. With cellulose substrates, the synergism between CBHI and endoglucanase I or II depended on the structural and ultrastructural features of the substrate. This effect, unrelated toendo-exocooperation, was found with substrates of intermediate crystallinity whereas weak or no synergism was recorded with cellulose microcrystals or the soluble carboxy-methyl cellulose derivative. Synergistic degradation of cellulose was also recorded with mixtures of CBHI and CBHII. On the other hand, synergism between endoglucanases and CBHII followed the pattern expected for anendo-exocooperation. These results presented support evidence for multiple types of cooperation between the cellulolytic enzymes.