Decreased Albumin and Increased Fibrinogen Secretion by Single Hepatocytes from Rats with Acute Inflammatory Reaction
Decreased Albumin and Increased Fibrinogen Secretion by Single Hepatocytes from Rats with Acute Inflammatory Reaction
复制标题
急性炎症反应大鼠单个肝细胞白蛋白减少和纤维蛋白原分泌增加
作者：
D. Bernuau;E. Rogier;G. Feldmann
During the acute inflammatory reaction (AIR), the plasma concentration of several plasma proteins, including fibrinogen, is increased as a result of enhanced production by the liver. Whether this increased production influences the production of the other plasma proteins at the single hepatocyte level is not known. Accordingly, we simultaneously measured albumin and fibrinogen secretion by single hepatocytes isolated from normal rats and from rats sacrificed 24 hr after a turpentine‐induced AIR, using an hemolytic plaque test. In normal rats, 74, 82, and 98% of the hepatocytes were detected as secreting albumin, after 1, 2, and 4 hr of incubation, respectively. Simultaneously, 0, 32, and 62% of the hepatocytes were detected as secreting fibrinogen at the same incubation times. For secretion of both proteins, a significant correlation was established between the diameter of a plaque‐forming hepatocyte and its hemolytic plaque. In rats with AIR, the formation of hemolytic plaques detecting albumin secretion was slowed down compared to normal rats, since only 0, 36, and 78% of the hepatocytes were forming hemolytic plaques after 1, 2, and 4 hr of incubation, respectively. Simultaneously, the formation of hemolytic plaques detecting fibrinogen secretion was increased compared to normal rats, since at the same incubation times, 52, 78, and 93% of the hepatocytes formed hemolytic plaques. A significant correlation between the diameter of a plaque‐forming hepatocyte and its hemolytic plaque was still maintained for both protein secretion.
DOI：
10.1073/pnas.78.8.4718
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
MORROW, JF;STEARMAN, RS;POTTER, DA
通讯作者：
POTTER, DA