Controlled vesicle deformation and lysis by single oscillating bubbles
Controlled vesicle deformation and lysis by single oscillating bubbles
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DOI：
10.1038/nature01613
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发表时间：
2003-05-08
期刊：
影响因子：
64.8
通讯作者：
Hilgenfeldt, S
中科院分区：
文献类型：
--
作者：
Marmottant, P;Hilgenfeldt, S
破裂（空化）气泡聚焦和聚集能量、力和应力的能力是空化损伤、声化学或声致发光等现象的根源(1,2)。在生物医学领域，超声波驱动的微泡已用于增强超声波图像的对比度(3)。气泡增强声穿孔 (4-6)（声学诱导膜破裂）的观察也为声穿孔作为细胞壁渗透技术（如电穿孔 (7) 和粒子枪 (8)）的替代疗法的治疗应用开辟了有趣的可能性。然而，这些开创性的实验未能查明剧烈破裂的气泡在膜上打开孔隙或更大孔的机制。在这里，我们提出了一个实验，其中温和的（线性）气泡振荡足以实现脂质膜的破裂。在这种情况下，可以精确控制气泡动力学和随后的声孔作用。使用微泡作为聚焦剂使微米级声学（微声学）成为一种可行的工具，可应用于细胞操作和细胞壁渗透以及微流体装置。
The ability of collapsing (cavitating) bubbles to focus and concentrate energy, forces and stresses is at the root of phenomena such as cavitation damage, sonochemistry or sonoluminescence(1,2). In a biomedical context, ultrasound-driven microbubbles have been used to enhance contrast in ultrasonic images(3). The observation of bubble-enhanced sonoporation(4-6)-acoustically induced rupture of membranes-has also opened up intriguing possibilities for the therapeutic application of sonoporation as an alternative to cell-wall permeation techniques such as electroporation(7) and particle guns(8). However, these pioneering experiments have not been able to pinpoint the mechanism by which the violently collapsing bubble opens pores or larger holes in membranes. Here we present an experiment in which gentle (linear) bubble oscillations are sufficient to achieve rupture of lipid membranes. In this regime, the bubble dynamics and the ensuing sonoporation can be accurately controlled. The use of microbubbles as focusing agents makes acoustics on the micrometre scale (microacoustics) a viable tool, with possible applications in cell manipulation and cell-wall permeation as well as in microfluidic devices.