Inhibitory Effects of Hydroxysafflor Yellow A on the Formation of Advanced Glycation End Products in Vitro
Inhibitory Effects of Hydroxysafflor Yellow A on the Formation of Advanced Glycation End Products in Vitro
复制标题
羟基红花黄 A 对体外晚期糖基化终末产物形成的抑制作用。
DOI：
10.1248/bpb.b12-00249
复制
发表时间：
2012-11-01
影响因子：
2
通讯作者：
Dai, Haibin
中科院分区：
文献类型：
--
作者：
Ni, Zhenzhen;Zhuge, Zhengbing;Dai, Haibin
目的：研究羟基红花黄色素A（HSYA）对体外蛋白质糖基化的抑制作用。采用牛血清白蛋白（BSA）-葡萄糖测定法、BSA-甲基乙二醛（MGO）测定法和N-乙酰甘氨酰-赖氨酸甲酯（G. K.）肽-核糖法检测HSYA的抑制作用。晚期糖基化终产物（AGEs）的产生通过AGEs特异性荧光和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）进行评估。在BSA-葡萄糖测定中，HSYA浓度依赖性地减少AGEs的形成，在1 mM时最大抑制作用为95%。此外，HSYA还对MGO介导的蛋白质修饰和随后的蛋白质交联具有显著的抑制作用。最后，当与G.K. HSYA具有抗糖基化作用，1 mM时最大抑制率为84%。总之，我们目前的研究提供了第一个证据HSYA对AGEs形成的体外抗糖化作用。
To investigate the inhibitory effects of hydroxysafflor yellow A (HSYA) on the protein glycation in vitro. Using bovine serum albumin (BSA)-glucose assay, BSA-methylglyoxal (MGO) assay, and N-acetylglycyl-lysine methyl ester (G.K.) peptide-ribose assay, inhibitory effects of HSYA were investigated. Advanced glycation end products (AGEs) production was assessed by AGEs-specific fluorescence and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). In BSA-glucose assay, HSYA concentration dependently decreased AGEs formation, with maximum inhibitory effects at 1 mM by 95%. Further more, HSYA also showed significant inhibitory effects on MGO-medicated protein modification and subsequent cross-linking of proteins. Finally, when co-incubated with G.K. peptide and ribose, HSYA exhibited its antiglycation effects, and the maximum inhibitory effects of HSYA at 1 mM were 84%. Overall, our present study provides the first evidence of the antiglycation effects of HSYA on AGEs formation in vitro.