STRUCTURE OF THE VOLTAGE-DEPENDENT POTASSIUM CHANNEL IS HIGHLY CONSERVED FROM DROSOPHILA TO VERTEBRATE CENTRAL NERVOUS SYSTEMS
STRUCTURE OF THE VOLTAGE-DEPENDENT POTASSIUM CHANNEL IS HIGHLY CONSERVED FROM DROSOPHILA TO VERTEBRATE CENTRAL NERVOUS SYSTEMS
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DOI：
10.1002/j.1460-2075.1988.tb03092.x
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发表时间：
1988-08-01
期刊：
影响因子：
11.4
通讯作者：
PONGS, O
中科院分区：
文献类型：
--
作者：
BAUMANN, A;GRUPE, A;PONGS, O
电压敏感性钾通道存在于脊椎动物和无脊椎动物的中枢神经系统中。我们用果蝇Shaker基因复合体探针交叉杂交，分离出一个大鼠脑cDNA。推导的蛋白质结构域的保守性表明，大鼠脑cDNA编码电压敏感性钾通道。在推导的氨基酸序列中，82%与果蝇Shaker蛋白同源，表明电压敏感性钾通道在进化过程中高度保守。选择压力是最高的序列面临的细胞内侧和跨膜段S4-S6，这表明这些领域是至关重要的电压依赖性钾通道功能。相应的大鼠mRNA显然属于优先在中枢神经系统中表达的mRNA分子家族。
Voltage-sensitive potassium channels are found in vertebrate and invertebrate central nervous systems. We have isolated a rat brain cDNA by cross-hybridization with a probe of the Drosophila Shaker gene complex. Structural conservation of domains of the deduced protein indicate that the rat brain cDNA encodes a voltage-sensitive potassium channel. Of the deduced amino acid sequence, 82% is homologous to the Drosophila Shaker protein indicating that voltage-sensitive potassium channels have been highly conserved during evolution. Selective pressure was highest on sequences facing the intracellular side and on proposed transmembrane segments S4-S6, suggesting that these domains are crucial for voltage-dependent potassium channel function. The corresponding rat mRNA apparently belongs to a family of mRNA molecules which are preferentially expressed in the central nervous system.