Identification of a ubiquitin family protein as a novel neutrophil chemotactic factor
Identification of a ubiquitin family protein as a novel neutrophil chemotactic factor
复制标题
DOI：
10.1016/j.bbrc.2003.08.038
复制
发表时间：
2003-09-26
影响因子：
3.1
通讯作者：
Kambara, H
中科院分区：
文献类型：
--
作者：
Owhashi, M;Taoka, Y;Kambara, H
中性粒细胞趋化性是一个过程，是必不可少的招聘中性粒细胞到发炎的网站。在本研究中，我们发现一个显着增加中性粒细胞趋化活性的红细胞（RBC）的小鼠感染鼠疟疾，约氏疟原虫的裂解物。用DE 52阴离子交换层析和Mono S阳离子交换层析相结合的方法从红细胞中分离出一种表观分子量为17 kDa的中性粒细胞趋化因子（IP 17）。对IP 17的N-末端氨基酸序列和MALDI-TOF质量分析的全面GenBank数据库搜索揭示，IP 17与ISG 15/UCRP的鼠同源物相同，ISG 15/UCRP是由干扰素β诱导的蛋白质的泛素家族的成员。重组小鼠ISG 15显示出与天然IP 17相当的中性粒细胞趋化活性。IP 17对中性粒细胞具有特异性趋化活性，并能诱导活化的中性粒细胞释放嗜酸性粒细胞趋化因子。这些结果表明，泛素家族蛋白ISG 15/UCRP在嗜中性粒细胞介导的免疫机制中具有新的功能。(C)2003年爱思唯尔公司All rights reserved.
Neutrophil chemotaxis is a process that is essential for the recruitment of neutrophils to an inflamed site. In the present study, we found a remarkable increase in neutrophil chemotactic activity in the lysate of red blood cells (RBC) of mice infected with murine malaria, Plasmodium yoelii. A neutrophil chemotactic factor with an apparent molecular weight of 17 kDa (IP17) was isolated from RBC by a combination of anion-exchange chromatography on DE52 and cation-exchange chromatography on Mono S. A comprehensive GenBank database search of N-terminal amino acid sequences and MALDI-TOF mass analysis of IP17 revealed that IP17 is identical to a murine homologue of ISG15/UCRP, a member of the ubiquitin family of proteins that are inducible by interferon-beta. Recombinant mouse ISG15 showed neutrophil chemotactic activity comparable to that of natural IP17. IP17 showed specific chemotactic activity forward neutrophils and activated neutrophils to induce the release of eosinophil chemotactic factors. These results suggest that the ubiquitin family protein ISG15/UCRP has novel functions in neutrophil-mediated immune mechanisms. (C) 2003 Elsevier Inc. All rights reserved.