Collagen formation by the hepatocyte in primary monolayer culture and in vivo.
Collagen formation by the hepatocyte in primary monolayer culture and in vivo.
复制标题
原代单层培养物和体内肝细胞形成胶原蛋白。
DOI：
10.1126/science.6828863
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Gay,S
中科院分区：
文献类型：
--
作者：
Diegelmann,RF;Guzelian,PS;Gay,R;Gay,S
免疫组化技术被用来确认生化证据，实质细胞分离成年大鼠肝脏，并保持在非复制单层培养2天合成IV型基底膜胶原。在无血清培养基中继续孵育时，肝细胞还合成I型和III型间质胶原。与培养中的这些结果一致，IV型胶原蛋白定位于病理大鼠肝切片中的肝细胞。因此，胶原蛋白的形成是以前未认识到的肝细胞的功能，可能是重要的肝纤维化或肝硬化的发病机制。
Immunohistochemical techniques were used to confirm biochemical evidence that parenchymal cells isolated from adult rat liver and maintained in nonreplicating monolayer culture for 2 days synthesized type IV basement membrane collagen. On continued incubation in serum-free medium, the hepatocytes also synthesized the interstitial collagens, types I and III. Consistent with these results in culture, type IV collagen was localized to the hepatocytes in slices of pathologic rat liver. Hence collagen formation is a previously unrecognized function of the hepatocyte that may be important in the pathogenesis of liver fibrosis or cirrhosis.