Telomere length, current perceived stress, and urinary stress hormones in women.
Telomere length, current perceived stress, and urinary stress hormones in women.
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DOI：
10.1158/1055-9965.epi-08-0614
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发表时间：
2009-02
期刊：
影响因子：
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通讯作者：
Sandler DP
中科院分区：
文献类型：
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作者：
Parks CG;Miller DB;McCanlies EC;Cawthon RM;Andrew ME;DeRoo LA;Sandler DP
端粒是覆盖和保护染色体末端的重复DNA序列；端粒过短可能导致细胞衰老或致癌转化。以前的发现表明，心理社会压力、端粒较短和慢性病风险之间存在联系。这项横断面研究在国家环境健康科学研究所姐妹研究的参与者样本(n=647)中检查了相对端粒长度与感知压力和尿液应激激素的关系，该研究是一组年龄在35岁至74岁之间的女性，她们的姐妹患有乳腺癌。用定量聚合酶链式反应测定白细胞平均端粒长度。使用感知应激量表和第一次晨尿中肌酸调节的神经内分泌激素来评估当前应激。线性回归模型估计了端粒长度碱基对(BP)的差异，这些差异与根据年龄、种族、吸烟和肥胖进行调整的压力测量相关。感受到较高压力的女性端粒略短[调整后的差异为−129个基点，处于或高于中等压力水平；95%可信区间(CI)，−292至33]，但端粒长度并不随压力水平的升高而单调减少。端粒较短与年龄增加(−27BP/年)、肥胖和当前吸烟独立相关。在55岁及以上的女性(−289bp；95%CI，−519至−59)、最近发生重大损失的女性(−420 BP；95%CI，−814至−27)以及尿儿茶酚胺高于平均水平的女性(如肾上腺素：−484BP；95%CI，−709至−259)中，端粒长度存在显著的应激相关差异。尽管在这一广泛的女性样本中，目前感知到的压力只与端粒较短有一定的相关性，但我们的发现表明，压力对端粒长度的影响可能会因神经内分泌反应、外部应激源和年龄而有所不同。
Telomeres are repetitive DNA sequences that cap and protect the ends of chromosomes; critically short telomeres may lead to cellular senescence or carcinogenic transformation. Previous findings suggest a link between psychosocial stress, shorter telomeres, and chronic disease risk. This cross-sectional study examined relative telomere length in relation to perceived stress and urinary stress hormones in a sample of participants (n = 647) in the National Institute of Environmental Health Sciences Sister Study, a cohort of women ages 35 to 74 years who have a sister with breast cancer. Average leukocyte telomere length was determined by quantitative PCR. Current stress was assessed using the Perceived Stress Scale and creatinine-adjusted neuroendocrine hormones in first morning urines. Linear regression models estimated differences in telomere length base pairs (bp) associated with stress measures adjusted for age, race, smoking, and obesity. Women with higher perceived stress had somewhat shorter telomeres [adjusted difference of −129bp for being at or above moderate stress levels; 95% confidence interval (CI), −292 to 33], but telomere length did not decrease monotonically with higher stress levels. Shorter telomeres were independently associated with increasing age (−27bp/year), obesity, and current smoking. Significant stress-related differences in telomere length were seen in women ages 55 years and older (−289bp; 95% CI, −519 to −59), those with recent major losses (−420bp; 95% CI, −814 to −27), and those with above-average urinary catecholamines (e.g., epinephrine: −484bp; 95% CI, −709 to −259). Although current perceived stress was only modestly associated with shorter telomeres in this broad sample of women, our findings suggest the effect of stress on telomere length may vary depending on neuroendocrine responsiveness, external stressors, and age.