The effect of co-culturing costal chondrocytes and dental pulp stem cells combined with exogenous FGF9 protein on chondrogenesis and ossification in engineered cartilage
The effect of co-culturing costal chondrocytes and dental pulp stem cells combined with exogenous FGF9 protein on chondrogenesis and ossification in engineered cartilage
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DOI：
10.1016/j.biomaterials.2012.07.020
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发表时间：
2012-11-01
期刊：
影响因子：
14
通讯作者：
Shen, Guofang
中科院分区：
文献类型：
--
作者：
Dai, Jiewen;Wang, Jia;Shen, Guofang
牙髓干细胞（DPSC），来自颅神经嵴细胞，是多能的，使它们成为组织工程的候选者，可能特别适用于颅面组织。肋软骨细胞（CC）可以很容易地获得，并表现出较高的初始细胞产量和扩增比关节软骨细胞。已发现CC保留软骨形成能力，可有效修复关节缺损。在这项研究中，人CCs与人DPSC共培养，结果表明，CCs能够提供软骨诱导龛，促进DPSC进行软骨分化，并增强软骨的形成。虽然单独的CC不能阻止软骨分化的DPSC的矿化，但CC与外源性FGF9组合能够同时促进DPSC的软骨形成并部分抑制其矿化。此外，FGF9可通过与FGFR3结合并增强DPSC中ERK 1/2的磷酸化来激活这种抑制。我们的研究结果强烈表明，CCs和DPSCs的共培养结合外源性FGF9可以同时促进软骨形成和部分抑制工程软骨的骨化。(c)2012爱思唯尔有限公司保留所有权利。
Dental pulp stem cells (DPSCs), which arise from cranial neural crest cells, are multipotent, making them a candidate for use in tissue engineering that may be especially useful for craniofacial tissues. Costal chondrocytes (CCs) can be easily obtained and demonstrate higher initial cell yields and expansion than articular chondrocytes. CCs have been found to retain chondrogenic capacity that can effectively repair articular defects. In this study, human CCs were co-cultured with human DPSCs, and the results showed that the CCs were able to supply a chondro-inductive niche that promoted the DPSCs to undergo chondrogenic differentiation and to enhance the formation of cartilage. Although CCs alone could not prevent the mineralization of chondro-differentiated DPSCs, CCs combined with exogenous FGF9 were able to simultaneously promote the chondrogenesis of DPSCs and partially inhibit their mineralization. Furthermore, FGF9 may activate this inhibition by binding to FGFR3 and enhancing the phosphorylation of ERK1/2 in DPSCs. Our results strongly suggest that the co-culture of CCs and DPSCs combined with exogenous FGF9 can simultaneously enhance chondrogenesis and partially inhibit ossification in engineered cartilage. (c) 2012 Elsevier Ltd. All rights reserved.