Peri-infarct flow transients predict outcome in rat focal brain ischemia.
Peri-infarct flow transients predict outcome in rat focal brain ischemia.
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DOI：
10.1016/j.neuroscience.2012.08.049
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发表时间：
2012-12-13
期刊：
影响因子：
3.3
通讯作者：
Greenberg, J. H.
中科院分区：
文献类型：
--
作者：
Lueckl, J.;Dreier, J. P.;Szabados, T.;Wiesenthal, D.;Bari, F.;Greenberg, J. H.
扩展性去极化伴随着脑血流量（CBF）的短暂变化。在先前研究的对照大鼠的事后分析中，我们分析了大鼠大脑中动脉闭塞后的CBF时程，以测试缺血内血流、再灌注和梗死周围血流瞬变（PIFT）的不同参数（幅度、数量）是否可以预测结果。用氟烷（n=23）或异氟烷（n=32）麻醉的Sprague-Dawley大鼠经历了90分钟的大脑中动脉细丝闭塞，然后再灌注72小时。用氯化三苯基四氮唑染色法测定梗死面积。通过激光多普勒血流仪在前囟外侧4-5 mm和后1-2 mm处监测相对CBF变化。进行了额外的细丝闭塞研究（n=12），以验证PIFT与扩展去极化的直流偏移耦合。PIFT-直流偏移研究显示，每个PIFT都与典型的负直流偏移相关，这是扩展去极化的特征。事后分析表明，PIFT的数量，特别是与缺血内血流水平的组合，可以预测皮质梗死的发展。这些发现表明，PIFT可以作为预测临床前动物研究结果的早期生物标志物。
Spreading depolarizations are accompanied by transient changes in cerebral blood flow (CBF). In a post-hoc analysis of previously studied control rats we analysed CBF time courses after middle cerebral artery occlusion in the rat in order to test whether intra-ischemic flow, reperfusion, and different parameters of peri-infarct flow transients (PIFTs) (amplitude, number) can predict outcome. Sprague-Dawley rats anesthetized with either halothane (n=23) or isoflurane (n=32) underwent 90-minute filament occlusion of the middle cerebral artery followed by 72 hours of reperfusion. The infarct size was determined by 2,3,5-triphenyltetrazolium chloride staining. Relative CBF changes were monitored by laser Doppler flowmetry at 4–5 mm lateral, and 1–2 mm posterior to Bregma. An additional filament occlusion study (n=12) was performed to validate that PIFTs were coupled to direct current shifts of spreading depolarization. The PIFT-direct current shift study revealed that every PIFT was associated with a negative direct current shift typical of spreading depolarization. Post-hoc analysis showed that the number of PIFTs, especially with the combination of intra-ischemic level of flow, can predict the development of cortical infarcts. These findings show that PIFTs can serve as an early biomarker in predicting outcome in preclinical animal studies.
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影响因子：
5.3
作者：
Ruscher, K;Freyer, D;Meisel, A
通讯作者：
Meisel, A
影响因子：
2.9
作者：
Burnett, MG;Detre, JA;Greenberg, JH
通讯作者：
Greenberg, JH
影响因子：
3.5
作者：
Durduran, Turgut;Zhou, Chao;Licht, Daniel J.
通讯作者：
Licht, Daniel J.
DOI：
10.1093/brain/awp102
发表时间：
2009-07
期刊：
Brain : a journal of neurology
影响因子：
--
作者：
Dreier JP;Major S;Manning A;Woitzik J;Drenckhahn C;Steinbrink J;Tolias C;Oliveira-Ferreira AI;Fabricius M;Hartings JA;Vajkoczy P;Lauritzen M;Dirnagl U;Bohner G;Strong AJ;COSBID study group
通讯作者：
COSBID study group
影响因子：
2.9
作者：
Dijkhuizen, RM;Beekwilder, JP;Nicolay, K
通讯作者：
Nicolay, K