A real-time documentation and mechanistic investigation of quantum dots-induced autophagy in live Caenorhabditis elegans.
A real-time documentation and mechanistic investigation of quantum dots-induced autophagy in live Caenorhabditis elegans.
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DOI：
10.1016/j.biomaterials.2015.08.044
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发表时间：
2015-12
期刊：
影响因子：
14
通讯作者：
Yanfeng Zhou;Qin Wang;Bin Song;Sicong Wu;Yuanyuan Su;Huimin Zhang;Yao He
中科院分区：
文献类型：
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作者：
Yanfeng Zhou;Qin Wang;Bin Song;Sicong Wu;Yuanyuan Su;Huimin Zhang;Yao He
自噬是细胞质中内源性或外源性物质降解的一个非常重要的细胞内过程。虽然纳米材料诱导的自噬已经被广泛研究，但关于纳米材料在生物体中诱导的自噬过程的实时信息仍然未知。本文以秀丽隐杆线虫（Caenorhabditis elegans）为模型生物，以荧光半导体量子点（QDs）为代表的纳米材料，系统地研究了量子点诱导的活体自噬现象。我们的研究结果表明，内化的量子点触发了秀丽隐杆线虫肠道细胞的完整自噬过程。进一步的研究表明，这种量子点诱导的自噬既不是对量子点释放的重金属离子的反应，也不是对外源量子点材料的吞噬，而是生物体清除和回收受损内体的一种防御策略。特别重要的是，我们首次提出了活体自噬体形成的实时跟踪，提供了这一过程的详细时空信息。该研究有助于我们更好地了解纳米材料与体内自噬的关系，并为纳米材料的安全性评价和生物应用提供宝贵的信息。
Autophagy is a highly important intracellular process for the degradation of endogenous or foreign contents in the cytoplasm. Though nanomaterials-induced autophagy has been extensively studied, real-time information about the autophagic process induced by nanomaterials in live organisms remains unknown. Here by using Caenorhabditis elegans as the model organism and fluorescent semiconductor quantum dots (QDs) as a representative nanomaterial, we systematically investigated the phenomenon of QDs-induced autophagy in live organisms. Our results demonstrated that the internalized QDs trigger a complete autophagic process inC. elegansintestinal cells. Further investigations revealed that this QD-induced autophagy inC. elegansis neither a response to released heavy metal ions by the QDs, nor an attempt to engulf exogenous QD materials, but a defensive strategy of the organism to clear and recycle damaged endosomes. Of particular significance, for the first time, we presented real-time tracking of autophagosomes formation in live organisms, providing detailed temporal-spatial information of this process. This study may help us better understand the relationship between nanomaterials and autophagyin vivo, and provide invaluable information for safety evaluation and bio-application of nanomaterials.