Evaluation of antibacterial and antifungal activity of new calcium-based cement (Biodentine) compared to MTA and glass ionomer cement.
Evaluation of antibacterial and antifungal activity of new calcium-based cement (Biodentine) compared to MTA and glass ionomer cement.
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DOI：
10.4103/0972-0707.148892
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发表时间：
2015-01
期刊：
影响因子：
--
通讯作者：
Reddy RB
中科院分区：
文献类型：
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作者：
Bhavana V;Chaitanya KP;Gandi P;Patil J;Dola B;Reddy RB
评估钙基水泥 Biodentine (Ca3SiO2) 与商用玻璃离子水门汀 (GIC) 和三氧化二矿物质骨料 (MTA) 的抗菌和抗真菌特性。制备 GIC、ProRoot MTA 和 Biodentine 颗粒，以测试这些水泥对四种口腔微生物菌株生长的影响：变形链球菌、粪肠球菌、大肠杆菌和白色念珠菌；采用琼脂扩散法。通过除去琼脂形成孔，并将处理过的材料立即放入孔中。将颗粒放置在接种板中，并在 37°C 孵育 24-72 小时后测量材料周围的生长抑制直径。使用方差分析（ANOVA）测试对数据进行分析，以比较不同浓度下三种水泥之间的差异。测试表明，Biodentine 对所有测试的微生物的抗菌活性都非常强，平均抑制区为 3.2 毫米，随着时间的推移，该抑制区向所有菌株延伸。对于 Biodentine、GIC 和 MTA，变形链球菌的抑菌圈直径显着大于粪肠球菌、念珠菌和大肠杆菌 (P < 0.05)。除 GIC 对念珠菌外，所有材料都对测试菌株表现出抗菌活性。观察到链球菌组具有最大的抑菌圈。 Biodentine 产生的抑制区比 MTA 和 GIC 更大。
To evaluate the antibacterial and antifungal properties of calcium-based cement, Biodentine (Ca3SiO2), compared to commercial glass ionomer cements (GICs) and mineral trioxide aggregate (MTA). Pellets of GICs, ProRoot MTA, and Biodentine were prepared to test the influence of these cements on the growth of four oral microbial strains: Streptococcus mutans, Enterococcus faecalis, Escherichia coli, and Candida albicans; using agar diffusion method. Wells were formed by removing the agar and the manipulated materials were immediately placed in the wells. The pellets were lodged in seeded plates and the growth inhibition diameter around the material was measured after 24-72 h incubation at 37°C. The data were analyzed using analysis of variance (ANOVA) test to compare the differences among the three cements at different concentrations. Test indicates that the antimicrobial activity of Biodentine, on all the microorganisms tested, was very strong, showing a mean inhibition zone of 3.2 mm, which extends over time towards all the strains. For Biodentine, GIC, and MTA, the diameters of the inhibition zones for S. mutans were significantly larger than for E. faecalis, Candida, and E. coli (P < 0.05). All materials showed antimicrobial activity against the tested strains except for GIC on Candida. Largest inhibition zone was observed for Streptococcus group. Biodentine created larger inhibition zones than MTA and GIC.