Double-quantum filtered rotational-echo double resonance
Double-quantum filtered rotational-echo double resonance
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DOI：
10.1016/j.jmr.2006.08.010
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发表时间：
2006-12-01
影响因子：
2.2
通讯作者：
Schaefer, Jacob
中科院分区：
文献类型：
--
作者：
Matsuoka, Shigeru;Schaefer, Jacob
在C-13{N-15}旋转回声双共振（REDOR）实验中，利用直接成键的C-13-C-13对的同核标量耦合建立了双量子滤波器（DQF），以去除C-13{N-15}的自然包舞背景。DQF标量和REDOR偶极演化周期重合，这对弱C-13-N-15偶极耦合的灵敏度有重要意义。计算和观察到的C-13{N-15} DQF-REDOR脱相与混合再结晶标记丙氨酸的测试样品一致。（CO）-C-13标记6 min后，采用C-13{N-15} DQF-REDOR方法定量测定单片均匀N-15大豆叶片的甘氨酸代谢，无背景干扰。(c) 2006爱思唯尔公司版权所有。
The homonuclear scalar coupling of a directly bonded C-13-C-13 pair has been used to create a double-quantum filter (DQF) to remove the natural-a bun dance C-13 background in C-13{N-15} rotational-echo double-resonance (REDOR) experiments. The DQF scalar and REDOR dipolar evolution periods are coincident which is important for sensitivity in the event of weak C-13-N-15 dipolar coupling. Calculated and observed C-13{N-15} DQF-REDOR dephasings were in agreement for a test sample of mixed recrystallized labeled alanines. Glycine metabolism in a single uniform-N-15 soybean leaf labeled for 6 min by (CO)-C-13, was measured quantitatively by C-13{N-15} DQF-REDOR with no background interferences. (c) 2006 Elsevier Inc. All rights reserved.