PGE2 binding sites and PG-stimulated cyclic AMP accumulation in rat isolated glomeruli and glomerular cultured cells
PGE2 binding sites and PG-stimulated cyclic AMP accumulation in rat isolated glomeruli and glomerular cultured cells
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大鼠离体肾小球和肾小球培养细胞中 PGE2 结合位点和 PG 刺激的环 AMP 积累
DOI：
10.1016/0303-7207(83)90048-5
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发表时间：
1983
影响因子：
4.1
通讯作者：
R. Ardaillou
中科院分区：
文献类型：
--
作者：
G. Friedlander;D. Chansel;J. Sraer;M. Bens;R. Ardaillou
[3H] pge2特异性结合离体肾小球。kdvalue为80 nM，位点数为528 fmol /mg。pge1和pge2对结合具有同等抑制作用，而pgi2的活性明显降低。在培养的肾小球系膜细胞和上皮细胞中不可能发现特异性的pge2受体。pge1、pge2和PGI2（0.1 ~ 100 μM）均能刺激离体肾小球和培养肾小球细胞的环AMP浓度。上皮细胞的基底环AMP高于系膜细胞和肾小球。在PGs存在的情况下，环AMP的积累在系膜细胞中最大。最大增产幅度在300-1400%之间。对于三种制剂，pge2和pge1的作用大于PGI2。pge1和PGE2的ed50值相同（上皮细胞和肾小球为5 μM，系膜细胞为20 μM）。pgi2的ed50值低于pge1或PGE2（肾小球、上皮细胞和系膜细胞分别为0.2、2和5 μM）。当在最大有效浓度下测试时，三种pg的影响不是相加的。这些结果表明，pge1、pge2和pgi2刺激肾小球和细胞的环AMP。[3H] pge2结合位点与这种生物学效应之间的关系尚未确定。继发于肾小球环AMP升高的生理事件也尚未确定。
[3H]PGE2specifically bound to isolated glomeruli. Thekdvalue and the number of sites were 80 nM and 528 fmoles/mg respectively. PGE1and PGE2resulted in equipotent inhibition of binding whereas PGI2was markedly less active. It was not possible to demonstrate specific receptors for PGE2in glomerular mesangial and epithelial cultured cells. pge1, PGE2and PGI2(0.1–100 μM) stimulated cyclic AMP concentration both in isolated glomeruli and glomerular cultured cells. Basal cyclic AMP in epithelial cells was greater than in mesangial cells or glomeruli. The cyclic AMP accumulation in the presence of PGs was greatest in mesangial cells. Maximum stimulation was in the range 300–1400%. For the three preparations, PGE2and PGE1produced a greater effect than PGI2. ED50values were identical for PGE1and PGE2(5 μM for epithelial cells and glomeruli, 20 μM for mesangial cells). ED50value for PGI2were lower than those for pge1or PGE2(0.2, 2 and 5 μM for glomeruli, epithelial cells and mesangial cells, respectively). The effects of the three PGs were not additive when tested at maximally effective concentrations. These results demonstrate that pge1, PGE2and PGI2stimulate glomerular and cellular cyclic AMP. A relationship between [3H]PGE2binding sites and this biological effect has not been established. The physiological events secondary to the increase in glomerular cyclic AMP are also yet to be determined.
培养的肾乳头集合管细胞中前列腺素、加压素和 cAMP 之间的相互关系。
DOI：
10.1016/0090-6980(82)90013-2
发表时间：
1982
期刊：
Prostaglandins
影响因子：
--
作者：
Grenier,FC;Allen,ML;Smith,WL
通讯作者：
Smith,WL