Chlorogenic Acid and Caffeine in Coffee Restore Insulin Signaling in Pancreatic Beta Cells.
Chlorogenic Acid and Caffeine in Coffee Restore Insulin Signaling in Pancreatic Beta Cells.
复制标题
DOI：
--
复制
发表时间：
2023-03
期刊：
影响因子：
--
通讯作者：
Y. Ihara;Shun-ichiro Asahara;Hiroyuki Inoue;M. Seike;Misaki Ando;Hiroki Kabutoya;Maki Kimura-Koyanagi;Y. Kido
中科院分区：
文献类型：
--
作者：
Y. Ihara;Shun-ichiro Asahara;Hiroyuki Inoue;M. Seike;Misaki Ando;Hiroki Kabutoya;Maki Kimura-Koyanagi;Y. Kido
据报道，经常喝咖啡的人患2型糖尿病的几率比不喝咖啡的人低。为了阐明咖啡预防2型糖尿病发病的机制，我们分析了咖啡中的咖啡因和绿原酸如何改变小鼠胰腺Β细胞系MIN6细胞中的胰岛素信号。结果表明，咖啡因改善内质网应激下的胰岛素信号传导，绿原酸通过cAMP反应元件结合蛋白增强胰岛素受体底物2的表达，促进胰岛素受体底物2下游的胰岛素信号传导，从而保护胰腺Β细胞。此外，绿原酸是一种有效的抗氧化剂，可以保护胰腺Β细胞。此外，体内和体外分析表明，绿原酸的胰腺Β细胞保护作用是通过减轻内质网应激介导的。结果表明，咖啡中的这些成分有可能减少2型糖尿病的发病机制，改善胰腺Β细胞功能不全。
The incidence of type 2 diabetes is reported to be lower in frequent coffee drinkers than in non-coffee drinkers. To elucidate the mechanism by which coffee prevents the onset of type 2 diabetes, we analyzed how caffeine and chlorogenic acid, which are components of coffee, alter insulin signaling in MIN6 cells, a mouse pancreatic Β cell line. The results showed that caffeine improved insulin signaling under endoplasmic reticulum stress, and chlorogenic acid protected pancreatic Β cells by enhancing the expression of insulin receptor substrate 2 via cAMP response element-binding protein and promoting insulin signaling downstream of insulin receptor substrate 2. In addition, chlorogenic acid was a potent antioxidant for the protection of pancreatic Β cells. Furthermore, in vivo and in vitro analyses revealed that the pancreatic Β cell-protective effect of chlorogenic acid was mediated by the alleviation of endoplasmic reticulum stress. The results suggest that these components of coffee have the potential to reduce the pathogenesis of type 2 diabetes and improve pancreatic Β cell insufficiency.