MYOSIN-II IS INVOLVED IN TRANSMITTER RELEASE AT SYNAPSES FORMED BETWEEN RAT SYMPATHETIC NEURONS IN CULTURE
MYOSIN-II IS INVOLVED IN TRANSMITTER RELEASE AT SYNAPSES FORMED BETWEEN RAT SYMPATHETIC NEURONS IN CULTURE
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DOI：
10.1016/0896-6273(94)90051-5
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发表时间：
1994-11-01
期刊：
影响因子：
16.2
通讯作者：
NONOMURA, Y
中科院分区：
文献类型：
--
作者：
MOCHIDA, S;KOBAYASHI, H;NONOMURA, Y
研究了肌球蛋白II在培养大鼠颈上神经节神经元间形成的胆碱能突触中的突触前功能。免疫荧光染色显示肌球蛋白II与突触体素共定位于突触前神经末梢。抗yosin II抗体注入突触前神经元抑制突触传递。肌球蛋白轻链激酶的两种抑制剂也以剂量依赖的方式抑制了传播：肽SM-1和有机抑制剂wortmannin。这些药物产生的抑制作用依赖于突触前活动。细胞外应用wortmannin也阻断突触传递，但其作用起效较慢。Wortmannin也降低完整颈上神经节的突触后电位和破伤风后增强。这些结果表明，肌凝蛋白轻链激酶磷酸化肌凝蛋白的模型，以及由此产生的肌动蛋白-肌凝蛋白相互作用的变化与神经递质释放有关。
The presynaptic function of myosin II was studied at cholinergic synapses formed between rat superior cervical ganglion neurons in culture. Immunofluorescent staining showed that myosin II was colocalized with synaptophysin at the presynaptic nerve terminals. Antimyosin II antibody introduced into presynaptic neurons inhibited synaptic transmission. Transmission was also inhibited in a dose-dependent manner by two inhibitors of myosin light chain kinase: a peptide, SM-1, and an organic inhibitor, wortmannin. The inhibition produced by these agents was dependent on presynaptic activity. Extracellularly applied wortmannin also blocked synaptic transmission, but its effects were slower in onset. Wortmannin also decreased postsynaptic potentials and post-tetanic potentiation in intact superior cervical ganglia. These results suggest a model in which myosin light chain kinase phosphorylates myosin, and the resultant change in actin-myosin interactions is involved in neurotransmitter release.