Effects of mutations near the bacteriochlorophylls in reaction centers from Rhodobacter sphaeroides.
Effects of mutations near the bacteriochlorophylls in reaction centers from Rhodobacter sphaeroides.
复制标题
球形红杆菌反应中心细菌叶绿素附近突变的影响。
DOI：
10.1021/bi00160a012
复制
发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
Allen,JP
中科院分区：
文献类型：
--
作者：
Williams,JC;Alden,RG;Murchison,HA;Peloquin,JM;Woodbury,NW;Allen,JP
方法设计突变体。在反应中心进行了一系列突变，其中每个Bchl附近的残基被另一个氨基酸取代，该氨基酸可能与V环的9-酮羰基形成氢键，受结构约束（表1）。因为这个羰基是共轭体系的一部分，氢的形成
METHODSDesign of Mutants. A set of mutations in the reaction center has been made in which residues near each Bchl were replaced with anotheramino acid that could possibly form a hydrogen bond to the 9-keto carbonyl of ring V, subject to the constraints of the structure (Table I). Since this carbonyl is part of the conjugation system, the formation of a hydrogen