Effect of complexation on the accumulation and elimination kinetics of cadmium and ciprofloxacin in the earthworm Eisenia fetida.
Effect of complexation on the accumulation and elimination kinetics of cadmium and ciprofloxacin in the earthworm Eisenia fetida.
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DOI：
10.1021/es104034g
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发表时间：
2011-05
影响因子：
11.4
通讯作者：
B. Wen;Rixiang Huang;Peng-ling Wang;Yanping Zhou;X. Shan;Shuzhen Zhang
中科院分区：
文献类型：
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作者：
B. Wen;Rixiang Huang;Peng-ling Wang;Yanping Zhou;X. Shan;Shuzhen Zhang
固体废物的土地利用会导致土壤中金属和抗菌剂的积累。为了解金属和抗生素相互作用对赤子爱胜蚓积累镉和环丙沙星的影响，测定了镉(Cd)和环丙沙星(CIP)的吸收、消除动力学和亚细胞分布。动力学符合一室一级动力学模型。Cd的添加不影响CIP的生物积累动力学和亚细胞分布。然而，Cd表现出不同的代谢和亚细胞分布模式。有CIP时，Cd吸收通量和消除速率常数分别是无CIP时的2.2倍和9.8倍。在CIP存在下，Cd从D组分(与颗粒有关)和E组分(与组织碎片和细胞膜有关)重新分布到C组分(与胞浆有关)。无CIP时，组分C中的Cd不能被排出，而有CIP时，组分C中的Cd被显著排出，排泄速率常数与CIP的排泄速率常数一致。蚯蚓体内CIP与Cd在摄取期和排除期之间存在良好的相关性(p<；0.01)。我们的结果表明CD-CIP复合体可以被蚯蚓摄取、储存和清除。
Land application of solid wastes leads to the accumulation of both metals and antimicrobials in soils. To understand the effects of metal and antibiotic interaction on their accumulation by the earthworm Eisenia fetida, uptake and elimination kinetics and subcellular distribution of cadmium (Cd) and ciprofloxacin (CIP) were determined. The kinetics was accurately described by a one-compartment first-order kinetic model. Bioaccumulation kinetics and subcellular distribution of CIP were not affected by Cd addition. However, Cd exhibited different metabolic and subcellular distribution patterns. With CIP, Cd uptake flux and elimination rate constants were about 2.2 and 9.8 times, respectively, those without CIP. In the presence of CIP, Cd redistributed from fractions D (associated with granules) and E (associated with tissue fragments and cell membranes) to fraction C (associated with cytosol). Without CIP, Cd in fraction C could not be excreted, whereas with CIP, Cd in fraction C was significantly excreted, and the excretion rate constant was consistent with that of CIP. A good relationship was found between CIP and Cd in earthworms during uptake and elimination periods (p < 0.01). Our results indicated that the Cd-CIP complex may be taken up, stored, and eliminated by earthworms.